I-optimal混匀实验设计制备肉桂叶饲用化复合食用菌菌剂

doi:10.3969/j.issn.0253-2417.2018.05.017

林产化学与工业 ›› 2018, Vol. 38 ›› Issue (5): 122-128.doi: 10.3969/j.issn.0253-2417.2018.05.017

• 研究报告 • 上一篇

I-optimal混匀实验设计制备肉桂叶饲用化复合食用菌菌剂

徐浩, 张宁, 杨静, 赵剑, 解静聪, 蒋剑春

中国林业科学研究院林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局林产化学工程重点开放性实验室;江苏省生物质能源与材料重点实验室, 江苏南京 210042

收稿日期:2018-05-31 出版日期:2018-10-25 发布日期:2018-11-06
通讯作者: 蒋剑春,研究员,博士,博士生导师,从事林产化学加工和生物质能源开发技术研究;E-mail:bio-energy@163.com。 E-mail:bio-energy@163.com
作者简介:徐浩(1988-),男,江苏南京人,助理工程师,硕士,主要从事生物质能源及工业微生物研究
基金资助:
中国林科院中央级公益性科研院所基本科研业务费专项资金（CAFYBB2017SY034）

I-Optimal Mixture Design of Compound Edible Fungi Inoculants for Bioconversion of Cinnamon Leaves into Feeding Stuff

XU Hao, ZHANG Ning, YANG Jing, ZHAO Jian, XIE Jingcong, JIANG Jianchun

Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab. for Biomass Chemical Utilization;Key and Open Lab. of Forest Chemical Engineering, SFA;Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China

Received:2018-05-31 Online:2018-10-25 Published:2018-11-06

摘要/Abstract

摘要： 为进一步提高肉桂叶发酵过程中各种饲用酶活性，通过I-optimal混匀设计考察了不同食用菌菌种间，以及食用菌与米曲霉间混合发酵产饲用酶活性的协同与拮抗效应。研究结果表明：特殊立方模型可以作为实验设计的回归模型，拟合结果可靠。在平菇、金针菇、木耳与米曲霉间，金针菇与木耳的菌丝产酶过程中存在拮抗效应，其余菌种生长过程中显示出明显的协同效应。通过数值优化算法得出2个最佳接种配比方案：配方一为17.6%木耳、44.4%平菇和38.0%米曲霉，对应的木聚糖酶酶活为9.14U/g、纤维素酶酶活为1.56U/g、植酸酶酶活为67.37U/g；配方二为60%金针菇和40%米曲霉，对应的木聚糖酶酶活为5.74U/g、纤维素酶酶活为1.34U/g、植酸酶酶活为58.39U/g。

关键词: 肉桂叶, 饲料, 食用菌, 米曲霉, 饲用酶

Abstract: To achieve higher enzymes activities during fermentation process of cinnamon leaves, the synergetic and antagonistic effects among different edible fungi, as well as between edible fungi and Aspergillus oryzae, were estimated by I-optimal mixture design. The results demonstratedthat special cubic models fit the mixture design methods well. Among Pleurotus ostreatus,Flammulina velutipes, Auricularia auricular and A. oryzae, antagonistic effects were found between F. velutipes and A.auricular during enzyme production, and synergistic effects were found among the rest species. Thus, by taking advantage of synergetic effects and avoiding of antagonistic effects, two formulas were made as following:A. auricular 17.6%, P. ostreatus 44.3% and A. oryzae 38% (9.14 U/g xylanase, 1.56 U/g cellulose, 67.37 U/g plant enzyme); or F. velutipes 60% and A. oryzae 40% (5.74 U/g xylanase, 1.34 U/g cellulase, 58.39 U/g plant enzyme).

Key words: cinnamon leaves, feeding stuff, edible fungi, Aspergillus oryzae, feed enzyme

中图分类号:

TQ35

徐浩, 张宁, 杨静, 赵剑, 解静聪, 蒋剑春. I-optimal混匀实验设计制备肉桂叶饲用化复合食用菌菌剂[J]. 林产化学与工业, 2018, 38(5): 122-128.

XU Hao, ZHANG Ning, YANG Jing, ZHAO Jian, XIE Jingcong, JIANG Jianchun. I-Optimal Mixture Design of Compound Edible Fungi Inoculants for Bioconversion of Cinnamon Leaves into Feeding Stuff[J]. Chemistry and Industry of Forest Products, 2018, 38(5): 122-128.

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I-optimal混匀实验设计制备肉桂叶饲用化复合食用菌菌剂

I-Optimal Mixture Design of Compound Edible Fungi Inoculants for Bioconversion of Cinnamon Leaves into Feeding Stuff

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