油橄榄叶提取物的制备及理化性质研究

doi:10.3969/j.issn.0253-2417.2019.04.012

林产化学与工业 ›› 2019, Vol. 39 ›› Issue (4): 83-90.doi: 10.3969/j.issn.0253-2417.2019.04.012

油橄榄叶提取物的制备及理化性质研究

王晓杰¹(),黄立新^1,*(),张彩虹^1,²,谢普军¹,邓叶俊¹,刘璐婕¹

1. 中国林业科学研究院林产化学工业研究所; 生物质化学利用国家工程实验室; 国家林业和草原局林产化学工程重点实验室; 江苏省生物质能源与材料重点实验室, 江苏南京 210042
2. 南京林业大学江苏省林业资源高效加工利用协同创新中心, 江苏南京 210037

收稿日期:2019-01-19 出版日期:2019-08-25 发布日期:2019-08-23
通讯作者: 黄立新 E-mail:xiaojie_wang@126.com;l_x_huang@163.com
作者简介:王晓杰(1992-),女,山东烟台人,硕士生,研究方向为农林产品深加工; E-mail:xiaojie_wang@126.com
基金资助:
国家自然科学基金青年基金项目(31800614)

Preparation and Physicochemical Properties of Olive Leaf Extract

Xiaojie WANG¹(),Lixin HUANG^1,*(),Caihong ZHANG^1,²,Pujun XIE¹,Yejun DENG¹,Lujie LIU¹

1. Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab. for Biomass Chemical Utilization; Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grassland Administration; Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China
2. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China

Received:2019-01-19 Online:2019-08-25 Published:2019-08-23
Contact: Lixin HUANG E-mail:xiaojie_wang@126.com;l_x_huang@163.com
Supported by:
国家自然科学基金青年基金项目(31800614)

摘要/Abstract

摘要：

对油橄榄叶中总多酚、总黄酮和橄榄苦苷热浸提工艺进行放大实验，考察了乙醇体积分数对活性物质提取效果的影响，并运用单指数衰减函数模型对提取过程进行动力学拟合。研究结果表明：80%乙醇提取效果最佳，提取液中总多酚、总黄酮和橄榄苦苷分别为3.77、2.85和3.03 g/L；单指数衰减函数拟合效果较好，R值均大于0.9，且随乙醇体积分数的升高，曲线拟合效果越好。在进风温度150℃、进料速率15 mL/min的条件下将脱除乙醇的提取液进行喷雾干燥处理，并运用AB-8大孔树脂对粗提物进行纯化后，总多酚质量分数由9.71%提高到31.65%，总黄酮质量分数由7.22%提高到10.98%，橄榄苦苷质量分数由9.16%提高到20.38%。对干燥后提取物粉末的含水量、孔隙率、密度、水溶性和吸湿性等理化性质进行测定，结果表明：不同体积分数乙醇提取物粉末含水量、密度和孔隙率相差不大，水提取物具有更好的水溶性（溶解度为98.38%），乙醇提取物的吸湿性均小于5%。

关键词: 油橄榄叶, 提取, 大孔树脂, 总多酚, 总黄酮, 橄榄苦苷

Abstract:

The scale-up experiment of hot reflux extraction of total polyphenols, total flavonoids and oleuropein from olive leaves was studied. The effect of ethanol concentration on the extraction of active substances was investigated, and the related kinetics was also evaluated by ExpDec1 model. The results showed the highest contents of total polyphenols, total flavonoids and oleuropein were obtained when the ethanol concentration was 80% and they were 3.77, 2.85 and 3.03 g/L, respectively. ExpDec1 model had a good fitting effect, and the fitting degree R value was higher than 0.9. The higher ethanol concentration, the better the fitness effect observed. Under the condition of inlet temperature 150℃ and feeding rate 15 mL/min, the extracts were dried by spray drying, and crude extracts were purified by AB-8 macroporous resin, the content of total polyphenols increased from 9.71% to 31.65%, the content of total flavonoids increased from 7.22% to 10.98%, and the content of oleuropein increased from 9.16% to 20.38%. The physicochemical properties of the extract powder were measured, such as moisture content, porosity, density, dissolution, moisture absorption, active substance, etc. The results showed that moisture content, density and porosity of different extracts were similar, water extract had better water solubility(98.38%), 80% ethanol extract had higher content of the active substance(the contents of total polyphenols, total flavonoids and oleuropein were 9.71%, 7.22% and 9.16%, respectively), and the contents of total flavonoids and oleuropein were about two times higher than that of other solvents.

Key words: olive leaves, extraction, macroporous resin, total polyphenols, total flavonoids, oleuropein

中图分类号:

TQ35
TS201.1

王晓杰,黄立新,张彩虹,谢普军,邓叶俊,刘璐婕. 油橄榄叶提取物的制备及理化性质研究[J]. 林产化学与工业, 2019, 39(4): 83-90.

Xiaojie WANG,Lixin HUANG,Caihong ZHANG,Pujun XIE,Yejun DENG,Lujie LIU. Preparation and Physicochemical Properties of Olive Leaf Extract[J]. Chemistry and Industry of Forest Products, 2019, 39(4): 83-90.

图/表 6

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活性物质 active compounds	提取溶剂 extraction solvents	模型拟合参数fitting parameters of models			R²
活性物质 active compounds	提取溶剂 extraction solvents	y₀	A₁	k₁	R²
总多酚 total polyphenols	H₂O	2.709 42	-1.883 62	1.594 11	0.988 40
	20% EtOH	2.635 21	-3.149 17	0.973 13	0.996 20
	50% EtOH	3.004 61	-4.966 44	0.818 93	0.984 22
	80% EtOH	3.670 18	-5.605 37	1.373 00	0.995 68
总黄酮 total flavonoids	H₂O	0.623 77	-0.487 55	1.283 85	0.930 58
	20% EtOH	0.903 74	-0.394 43	1.442 63	0.983 00
	50% EtOH	1.730 31	-0.892 99	1.287 22	0.981 09
	80% EtOH	2.902 72	-5.808 09	1.175 29	0.997 74
橄榄苦苷 oleuropein	H₂O	1.434 81	-0.769 68	2.249 48	0.984 40
	20% EtOH	1.885 04	-2.209 87	1.233 17	0.992 24
	50% EtOH	1.824 57	-0.409 69	1.358 00	0.998 91
	80% EtOH	2.997 99	-6.435 99	1.029 46	0.999 30

溶剂 solvent	时间/h time	总多酚total polyphenols			总黄酮total flavonoids			橄榄苦苷oleuropein
溶剂 solvent	时间/h time	W_P	W_M	R	W_P	W_M	R	W_P	W_M	R
H₂O	2.5	2.21	2.11	0.95	0.55	0.55	0.99	1.18	1.16	0.99
H₂O	7.5	2.67	2.41	0.90	0.62	0.61	0.98	1.41	1.38	0.98
20% EtOH	2.5	2.39	2.20	0.92	0.83	0.77	0.93	1.59	1.54	0.97
20% EtOH	7.5	2.63	2.57	0.98	0.90	0.89	0.99	1.88	1.79	0.95
50% EtOH	2.5	2.77	3.0	0.91	1.60	1.76	0.91	1.76	1.68	0.95
50% EtOH	7.5	3.00	3.31	0.91	1.73	1.91	0.91	1.82	1.88	0.97
80% EtOH	2.5	2.76	3.02	0.92	2.21	2.30	0.96	2.43	2.49	0.98
80% EtOH	7.5	3.65	3.58	0.98	2.89	2.86	0.99	2.99	3.13	0.96

%
提取溶剂 extraction solvent	总多酚total polyphenols		总黄酮total flavonoids		橄榄苦苷oleuropein
提取溶剂 extraction solvent	质量分数 mass fraction	损失率 loss rate	质量分数 mass fraction	损失率 loss rate	质量分数 mass fraction	损失率 loss rate
H₂O	8.04±0.34	52	2.80±0.01	31	3.93±0.27	54
20% EtOH	8.56±0.24	56	3.26±0.05	46	4.26±0.24	54
50% EtOH	9.37±0.38	48	4.40±0.09	54	5.35±0.29	54
80% EtOH	9.71±0.05	55	7.22±0.02	55	9.16±0.21	53

提取溶剂¹⁾ extraction solvent	含水量/% moisture content	真密度/(g·mL^-1) true density	体积密度/(g·mL^-1) bulk density	孔隙率 porosity	吸湿量/% hygroscopicity	水溶性/% water solubility
H₂O	1.01	1.256	0.476	0.621	8.09	98.38
20% EtOH	0.74	1.205	0.445	0.631	3.21	88.58
50% EtOH	0.68	1.324	0.465	0.648	2.60	88.38
80% EtOH	0.86	1.343	0.489	0.636	3.07	64.18
80%EtoH^*	0.75	1.356	0.476	0.648	2.91	77.40

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油橄榄叶提取物的制备及理化性质研究

Preparation and Physicochemical Properties of Olive Leaf Extract

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