两步法预处理对杨木酶水解及木质素吸附性能的影响

doi:10.3969/j.issn.0253-2417.2019.06.009

林产化学与工业 ›› 2019, Vol. 39 ›› Issue (6): 68-74.doi: 10.3969/j.issn.0253-2417.2019.06.009

两步法预处理对杨木酶水解及木质素吸附性能的影响

储秋露¹(),陈雪艳¹,宋凯^2,*(),王静³,张晓东¹,施爱平¹

1. 江苏大学农业装备工程学院, 江苏镇江 212013
2. 南京林业大学生物与环境学院, 江苏南京 210037
3. 广东省林业科学研究院, 广东广州 510520

收稿日期:2019-06-06 出版日期:2019-12-28 发布日期:2019-12-21
通讯作者: 宋凯 E-mail:Mina_chu@ujs.edu.cn;ksong@njfu.edu.cn
作者简介:储秋露(1986— ),女,江苏宜兴人,副研究员,主要从事生物质转化的研究; E-mail:Mina_chu@ujs.edu.cn
基金资助:
江苏省高校优势学科项目(无编号);江苏大学高级技术人才科研启动基金资助项目(17JDG034);国家自然科学基金资助项目(31901271)

Effects of Two-stage Pretreatment on Enzymatic Hydrolysis of Poplar and Adsorption Performance of Lignin

Qiulu CHU¹(),Xueyan CHEN¹,Kai SONG^2,*(),Jing WANG³,Xiaodong ZHANG¹,Aiping SHI¹

1. School of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang 212013, China
2. College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China
3. Guangdong Academy of Forestry, Guangzhou 510520, China

Received:2019-06-06 Online:2019-12-28 Published:2019-12-21
Contact: Kai SONG E-mail:Mina_chu@ujs.edu.cn;ksong@njfu.edu.cn
Supported by:
江苏省高校优势学科项目(无编号);江苏大学高级技术人才科研启动基金资助项目(17JDG034);国家自然科学基金资助项目(31901271)

摘要/Abstract

摘要：

研究了不同的两步法预处理对杨木酶水解和木质素吸附性能的影响，结果显示：未处理原料中木质素为29.05%，其酶水解得率仅为15.24%；蒸汽爆破一步法预处理后物料中木质素为34.88%，酸性基团仅为10.16 mmol/kg，酶水解得率为56.88%，预处理过程中木质素几乎没有脱除，因此未能回收作为吸附剂使用。碱性氧化-蒸汽爆破和碱性磺化-蒸汽爆破两步法预处理后物料中木质素减少至21.06%和17.68%，酸性基团增加至101.34和107.69 mmol/kg，酶水解得率由一步法的56.88%提高至74.38%和81.09%，两步法预处理脱除了原料中50%左右的木质素，经回收可作为重金属离子吸附剂使用，对Pb（Ⅱ）的最大吸附量分别为158.73和142.86 mg/g。分析表明：碱性磺化-蒸汽爆破两步法预处理既可大量脱除木质素，增强纤维素酶水解，又可对木质素进行改性，提高木质素对重金属离子Pb（Ⅱ）的吸附性能。

关键词: 杨木, 预处理, 酶水解, 木质素, 吸附

Abstract:

Effects on enzymatic hydrolysis of poplar and adsorption performance of lignin by two-stage pretreatment were established. The lignin content of raw biomass was 29.05%, while cellulose hydrolysis yield was only 15.24%. After steam explosion pretreatment, lignin content increased to 34.88%, and the acid group in biomass was 10.16 mmol/kg. As a result, the yield of cellulose by hydrolysis enhanced to 56.88%. However, lignin was hardly removed from biomass, which could not be recovered by acid precipitation to prepare lignin adsorbents.The lignin content decreased to 21.06% and 17.68%, incorporated with acid groups increased to 101.34 and 107.69 mmol/kg after alkaline oxidation-steam explosion and alkaline sulfonation-steam explosion pretreatment.And the yield of cellulose hydrolysis enhanced from 56.88% to 74.38% and 81.09%. Besides, around 50% of the original lignin was removed from biomass, which could be recovered as lignin adsorbents for Pb(Ⅱ) ions with adsorption capacity of 158.73 and 142.86 mg/g. The obtained results demonstrated that alkaline sulfonation-steam explosion pretreatment largely improved lignin removal and modification, which enhanced both the enzymatic hydrolysis of cellulose and the adsorption performance of lignin for holistic utilization of lignocellulosic biomass.

Key words: poplar, pretreatment, enzymatic hydrolysis, lignin, adsorption

中图分类号:

TQ35

储秋露,陈雪艳,宋凯,王静,张晓东,施爱平. 两步法预处理对杨木酶水解及木质素吸附性能的影响[J]. 林产化学与工业, 2019, 39(6): 68-74.

Qiulu CHU,Xueyan CHEN,Kai SONG,Jing WANG,Xiaodong ZHANG,Aiping SHI. Effects of Two-stage Pretreatment on Enzymatic Hydrolysis of Poplar and Adsorption Performance of Lignin[J]. Chemistry and Industry of Forest Products, 2019, 39(6): 68-74.

图/表 5

表1

表2

表3

图1

表4

参考文献 15

1	惠珍珍, 迟聪聪, 柳咪, 等. 乙酸预处理对木质纤维素结构及性能的影响[J]. 林业工程学报, 2019, 4 (1): 88- 93.
	HUI Z Z , CHI C C , LIU M , et al. Effect of acetic acid pretreatment on structure and properties of lignocellulose material[J]. Journal of Forestry Engineering, 2019, 4 (1): 88- 93.
2	朱圆圆, 顾夕梅, 朱均均, 等. 两步碱法预处理对玉米秸秆组分及结构的影响[J]. 中国科技论文, 2015, 10 (12): 1376- 1381. doi: 10.3969/j.issn.2095-2783.2015.12.006
	ZHU Y Y , GU X M , ZHU J J , et al. Effects of two-step alkaline pretreatment on components and structure of corn stover[J]. China Sciencepaper, 2015, 10 (12): 1376- 1381. doi: 10.3969/j.issn.2095-2783.2015.12.006
3	王敬枫, 郝茜珣, 侯新村, 等. 碱性亚硫酸盐预处理对杂交狼尾草酶水解特性的影响[J]. 林业工程学报, 2018, 3 (2): 95- 101.
	WANG J F , HAO X X , HOU X C , et al. Effect of alkaline sulfite pretreatment on enzymatic hydrolysis of hybrid China wolftailgrass[J]. Journal of Forestry Engineering, 2018, 3 (2): 95- 101.
4	TIAN D , HU J G , CHANDRA R P , et al. Valorizing recalcitrant cellulolytic enzyme lignin via lignin nanoparticles fabrication in an integrated biorefinery[J]. ACS Sustainable Chemistry and Engineering, 2017, 5 (3): 2702- 2710. doi: 10.1021/acssuschemeng.6b03043
5	沈王庆, 雷阳. NaOH/KOH改性柠檬渣吸附Pb²⁺的动力学和热力学研究[J]. 林产化学与工业, 2017, 37 (3): 141- 146. doi: 10.3969/j.issn.0253-2417.2017.03.020
	SHEN W Q , LEI Y . Kinetics and thermodynamics studies of the adsorption of Pb²⁺ onto NaOH/KOH modified lemon residues[J]. Chemistry and Industry of Forest Products, 2017, 37 (3): 141- 146. doi: 10.3969/j.issn.0253-2417.2017.03.020
6	应文俊, 吴凯, 史正军, 等. 磷酸联合过氧化氢预处理玉米芯及其酶水解效率的影响[J]. 林产化学与工业, 2018, 38 (5): 100- 106. doi: 10.3969/j.issn.0253-2417.2018.05.014
	YING W J , WU K , SHI Z J , et al. Pretreatment of corn cob by the combination of H₃PO₄-H₂O₂ and their effect on enzymatic hydrolysis efficiency[J]. Chemistry and Industry of Forest Products, 2018, 38 (5): 100- 106. doi: 10.3969/j.issn.0253-2417.2018.05.014
7	SLUITER A, HAMES B, RUIZ R, et al.Deter mination of structural carbohydrates and lignin in biomass: Laboratory Analytical Procedure(LAP): NREL/TP-510-42618[G]. Golden: National Renewable Energy Laboratory, 2012 - 08 - 03.
8	KATZ S , BEATSON R P , SCALLAN A M . The determination of strong and weak acidic groups in sulfite pulps[J]. Svensk Papperstidning, 1984, 87 (6): 48- 53.
9	CHANDRA R , EWANICK S , HSIEH C , et al. The characterization of pretreated lignocellulosic substrates prior to enzymatic hydrolysis.Part 1:A modified Simons' stainining technique[J]. Biotechnology Progress, 2008, 24, 1178- 1185. doi: 10.1002/btpr.33
10	王琰, 朱杨苏, 勇强, 等. 酶水解pH值对酸性亚硫酸氨盐预处理杨木糖化效率的影响[J]. 纤维素科学与技术, 2015, 23 (3): 15- 21.
	WANG Y , ZHU Y S , YONG Q , et al. Effects of pH on the enzymatic saccharification of acid sodium bisulfite pretreated poplar[J]. Journal of Cellulose Science and Technology, 2015, 23 (3): 15- 21.
11	李辉勇, 黄可龙, 金密, 等. 稻草秸秆的碱性臭氧预处理效果[J]. 农业工程学报, 2010, 26 (4): 264- 268. doi: 10.3969/j.issn.1002-6819.2010.04.045
	LI H Y , HUANG K L , JIN M , et al. Effects of alkaline ozone pretreatment on rice straw[J]. Transactions of the Chinese Society of Agricultural Engineering, 2010, 26 (4): 264- 268. doi: 10.3969/j.issn.1002-6819.2010.04.045
12	CHU Q L , SONG K , HU J G , et al. Integrated process for the coproduction of fermentable sugars and lignin adsorbents from hardwood[J]. Bioresource Technology, 2019, 289, 121- 659.
13	秦书百川, 刘彦涛, 卜令习, 等. 碱性亚硫酸盐耦合低压蒸汽预处理慈竹及其纤维素酶解转化[J]. 北京林业大学学报, 2016, 38 (7): 98- 104.
	QIN S B C , LIU Y T , BU L X , et al. Alkaline sulfite and low-pressure steam coupled pretreatment on Neosinocalamus affinis and its enzymatic hydrolysis[J]. Journal of Beijing Forestry University, 2016, 38 (7): 98- 104.
14	HUANG C X , MA J M , LIANG C , et al. Influence of sulfur dioxide-ethanol-water pretreatment on the physicochemical properties and enzymatic digestibility of bamboo residues[J]. Bioresource Technology, 2018, 263, 17- 24. doi: 10.1016/j.biortech.2018.04.104
15	CHEN H C , NI L , QU X L , et al. The effect of hot water pretreatment on the heavy metal adsorption capacity of acid insoluble lignin from Paulownia elongate[J]. Journal of Chemical Technology and Biotechnology, 2018, 93 (4): 1105- 1112. doi: 10.1002/jctb.5469

预处理方法 pretreatment	化学组成chemical composition/%			回收率recovery/%		木质素脱除率/% lignin removal
预处理方法 pretreatment	纤维素 cellulose	半纤维素 hemicellulose	木质素 lignin	纤维素 cellulose	半纤维素 hemicellulose	木质素脱除率/% lignin removal
原料raw material	46.65	20.26	29.05	—	—	—
蒸汽爆破steam explosion	54.51	6.27	34.88	94.63	51.30	4.32
碱处理-蒸汽爆破 alkaline treatment-seteam explosion	58.22	16.01	22.87	95.09	75.24	40.29
碱性氧化-蒸汽爆破 alkaline oxidation-steam explosion	59.88	16.21	21.06	93.63	73.02	47.18
碱性磺化-蒸汽爆破 alkaline sulfonation-steam explosion	61.23	18.58	17.68	95.27	78.36	56.03

预处理方法 pretreatment	酸性基团/(mmol·kg^-1) acid groups	纤维素可及度/(mg·g^-1) cellulose accessibility	酶水解得率/% enzymatic hydrolysis	糖得率/% sugar yield
原料raw material	46.67	52.58	15.24	11.61
蒸汽爆破steam explosion	10.16	106.44	56.88	50.69
碱处理-蒸汽爆破 alkaline treatment-seteam explosion	29.99	97.65	67.08	61.38
碱性氧化-蒸汽爆破 alkaline oxidation-steam explosion	101.34	119.10	74.38	66.09
碱性磺化-蒸汽爆破 alkaline sulfonation-steam explosion	107.69	132.04	81.09	73.71

预处理方法 pretreatment methods	木质素回收率/% lignin recovery	阿拉伯糖/% arabinose	葡萄糖/% glucose	木糖/% xylose
碱处理-蒸汽爆破 alkali treatment-steam explosion	78.31	0.10	0.23	2.49
碱性氧化-蒸汽爆破 alkaline oxidation-steam explosion	82.72	0	0.89	6.16
碱性磺化-蒸汽爆破 alkaline sulfonation-steam explosion	86.80	0.08	0.01	2.40

样品 samples	q_{e, exp}/ (mg·g^-1)	准一级动力学 pseudo-first-order kinetics			准二级动力学 pseudo-second-order kinetics			Langmuir方程 Langmuir isotherm
样品 samples	q_{e, exp}/ (mg·g^-1)	R²	k₁/min^-1	q_e/ (mg·g^-1)	R²	k₂/ (g·mg^-1·min^-1)	q_e/ (mg·g^-1)	R²	q_m/ (mg·g^-1)	b/ (L·mg^-1)
Kraft木质素Kraft lignin	25.23	0.9878	0.10	22.56	0.9997	0.0089	26.53	0.9999	48.78	0.0126
碱木质素alkali lignin	53.32	0.9850	0.17	56.05	0.9998	0.0079	54.95	0.9998	93.46	0.0138
碱性氧化木质素 alkaline oxidation lignin	61.60	0.9193	0.13	35.08	1.0000	0.0081	63.69	0.9996	158.73	0.0104
碱性磺化木质素 alkaline sulfonation lignin	59.07	0.9731	0.12	43.56	0.9999	0.0080	60.24	0.9982	142.86	0.0131

[1]	卢传巍, 郭晓亮, 蔡青云, 王春鹏, 储富祥, 王基夫. 甲基丙烯酸化木质素磺酸钙/聚丙烯酰胺复合水凝胶的制备与性能研究[J]. 林产化学与工业, 2019, 39(6): 75-80.
[2]	李宁, 陈智糠, 张一甫. 酚化木质素改性酚醛树脂胶黏剂的制备及性能表征[J]. 林产化学与工业, 2019, 39(6): 95-101.
[3]	魏录录, 严振宇, 邓拥军, 韩善明, 梁龙, 房桂干. 挤压预处理对杨木APMP浆纤维形态及制浆性能的影响[J]. 林产化学与工业, 2019, 39(6): 109-114.
[4]	黄晨, 武新星, 赖晨欢, 黄曹兴, 李鑫, 勇强. 高灰分麦糠水热预处理及其酶水解性能研究[J]. 林产化学与工业, 2019, 39(5): 26-32.
[5]	陈雪艳, 储秋露, 王静, 张晓东, 施爱平. 两步法预处理脱除木质素对杨木酶水解的影响[J]. 林产化学与工业, 2019, 39(5): 73-79.
[6]	王兴佳, 乔炜, 肖达明, 李淑君. 铝氧单钠固体超强碱催化降解木质素的研究[J]. 林产化学与工业, 2019, 39(5): 80-86.
[7]	张浩楠, 韦家慧, 赵辉, 李丹, 任浩. 浒苔纤维聚丙烯酸吸水树脂的制备及性能分析[J]. 林产化学与工业, 2019, 39(5): 101-107.
[8]	陈金伟,王丹,商士斌,刘鹤,蔡照胜. 端氨基超支化聚合物接枝双醛基纳米纤维素的制备及其对Ni²⁺的吸附[J]. 林产化学与工业, 2019, 39(4): 18-26.
[9]	张清桐,运晓静,颜德鹏,李明富,王双飞,闵斗勇. 太阳光催化木质素制备银纳米颗粒[J]. 林产化学与工业, 2019, 39(4): 35-41.
[10]	吴少基,江坤,叶跃元,刘运权,王夺,李水荣. 污泥与杨木屑共热解焦制活性炭及其废水处理应用[J]. 林产化学与工业, 2019, 39(4): 56-64.
[11]	王鹏飞,任浩,翟华敏. 杨木碳水化合物自水解特征的P因子调控[J]. 林产化学与工业, 2019, 39(4): 65-71.
[12]	孙永昌,张冰清,刘力鸣,刘肖南,史正军. 木质素基炭材料的制备及其对Cr(Ⅵ)的吸附机制研究[J]. 林产化学与工业, 2019, 39(4): 120-128.
[13]	马红亮,陈健,焦健,邓拥军,孔振武,房桂干. 长链硅烷改性杨木纤维的制备及其表征[J]. 林产化学与工业, 2019, 39(3): 25-33.
[14]	陈超,王傲,孙康,卢辛成,许伟,蒋剑春. 生物质原料的Ni催化石墨化研究[J]. 林产化学与工业, 2019, 39(3): 94-100.
[15]	李延庆,刘志明,程小凯,曹梦楠,王佳楠. 海藻酸钠/纤维素复合微球的制备及性能表征[J]. 林产化学与工业, 2019, 39(2): 67-72.

两步法预处理对杨木酶水解及木质素吸附性能的影响

Effects of Two-stage Pretreatment on Enzymatic Hydrolysis of Poplar and Adsorption Performance of Lignin

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 15

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

投稿指南

在线期刊

相关单位

联系我们