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林产化学与工业 ›› 2020, Vol. 40 ›› Issue (1): 68-76.doi: 10.3969/j.issn.0253-2417.2020.01.010

• 研究报告 • 上一篇    下一篇

杜仲籽油脂质体的制备及其稳定性研究

陈亮1,2(),詹桂萍2,鲁颖1,李加兴1,2,余佶2,麻成金1,2,姚茂君1,2,*()   

  1. 1. 吉首大学 林产化工工程湖南省重点实验室, 湖南 张家界 427000
    2. 吉首大学 食品科学研究所, 湖南 吉首 416000
  • 收稿日期:2019-07-11 出版日期:2020-02-28 发布日期:2020-03-06
  • 通讯作者: 姚茂君 E-mail:936785438@qq.com;yaomaojun@126.com
  • 作者简介:陈亮(1994-),男,浙江杭州人,硕士生,主要从事天然生物与功能性食品的开发与利用; E-mail:936785438@qq.com
  • 基金资助:
    湖南省科技厅重点研发计划资助项目(2018NK2045)

Preparation and Stability of Eucommia ulmoides Oliv.Seed Oil Liposomes

Liang CHEN1,2(),Guiping ZHAN2,Ying LU1,Jiaxing LI1,2,Ji YU2,Chengjin MA1,2,Maojun YAO1,2,*()   

  1. 1. Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China
    2. Institute of Food Science, Jishou University, Jishou 416000, China
  • Received:2019-07-11 Online:2020-02-28 Published:2020-03-06
  • Contact: Maojun YAO E-mail:936785438@qq.com;yaomaojun@126.com
  • Supported by:
    湖南省科技厅重点研发计划资助项目(2018NK2045)

摘要:

采用乙醇注入-超声波法制备杜仲籽油脂质体,在单因素的基础上,通过Plackett-Burman试验、最陡爬坡试验和响应面试验优化制备条件,并对其稳定性进行考察。脂质体制备的最佳工艺条件为:大豆卵磷脂240 mg,大豆卵磷脂与β-谷甾醇质量比(X1)为3.1:1,大豆卵磷脂与杜仲籽油质量比(X2)为4:1,吐温-80用量(X3)为20%(以大豆卵磷脂质量计),PBS缓冲液pH值(X4)为6.9,超声波作用时间(X5)为13 min,超声波功率(X6)为180 W。在此优化工艺条件下,杜仲籽油脂质体包埋率达75.26%,平均粒径为137.4 nm,Zeta电位为-19.3 mV,多分散系数(PDI)为0.219。在透射电镜下观察到杜仲籽油脂质体呈准球形且外观平滑完整,分布均匀,无明显塌陷和凝聚成团现象。稳定性试验结果发现:杜仲籽油脂质体稳定性受温度影响较大,4℃下各项指标稳定性均优于25和40℃;在4℃下储藏15天时,包埋率由75.26%下降到61.45%,pH值略微下降,平均粒径由137.4 nm上升到160.9 nm,丙二醛(MDA)由0.023 2 mg/g上升到0.033 1 mg/g。

关键词: 杜仲籽油, 脂质体, 包埋率, 响应面, 稳定性

Abstract:

Eucommia ulmoides Oliv. seed oil liposomes were prepared by a combined technology of ethanol injection and ultrasound, and the stability of liposomes was also evaluated. The optimized conditions and the encapsulation efficiency of E. ulmoides seed oil liposomes were investigated by Plackett-Burman design, the steepest ascent design and response surface methodology.The results showed that the optimal conditions selected were as follows:the mass ratio of phosphatidylcholine to sitosterol (X1)3.1:1, the mass ratio of phosphatidylcholine to E. ulmoides seed oil (X2) 4:1, dosage of tween-80(X3) 20%, phosphate buffer solution pH value (X4) 6.9, ultrasonic time (X5) 13 min, ultrasonic power (X6) 180 W. Under this condition, the actual encapsulation efficiency, average particle size, average potential and poly dispersity index (PDI) of E. ulmoides seed oil liposomes were 75.26%, 137.4 nm, -19.3 mV and 0.219, respectively. The particles showed irregular oval shape and smooth and complete morphology via the transmission electron microscopy observation. The stability experiments showed that E. ulmoides seed oil liposomes were significantly affected by the temperature, and the stability of all indexes at 4℃ was better than that at 25℃ and 40℃.When the E. ulmoides seed oil liposomes were stored at 4℃ for 15 days, the encapsulation efficiency decreased from 75.26% to 61.45%, the pH value decreased slightly, the average particle size increased from 137.4 nm to 160.9 nm, and malondialdehyde (MDA) increased from 0.023 2 mg/g to 0.033 1 mg/g.

Key words: Eucommia ulmoides seed oil, liposomes, encapsulation efficiency, response surface methodology, stability

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