春秋两季油樟叶的油细胞形态、精油成分及其抗氧化活性研究

doi:10.3969/j.issn.0253-2417.2021.01.006

林产化学与工业 ›› 2021, Vol. 41 ›› Issue (1): 38-44.doi: 10.3969/j.issn.0253-2417.2021.01.006

春秋两季油樟叶的油细胞形态、精油成分及其抗氧化活性研究

程贤¹(), 毕良武¹^,*(), 李胜男¹, 陈玉湘¹, 赵振东¹, 莫开林²

1. 中国林业科学研究院林产化学工业研究所; 生物质化学利用国家工程实验室; 国家林业和草原局林产化学工程重点实验室; 江苏省生物质能源与材料重点实验室; 江苏省林业资源高效加工利用协同创新中心, 江苏南京 210042
2. 四川省林业科学研究院, 四川成都 610081

收稿日期:2020-11-10 出版日期:2021-02-28 发布日期:2021-03-03
通讯作者: 毕良武 E-mail:chengx9013@163.com;biliangwu@126.com
作者简介:毕良武, 研究员, 博士, 博士生导师, 主要从事天然产物化学与利用研究; E-mail: biliangwu@126.com
程贤(1990-), 女, 山东济宁人, 助理研究员, 博士, 主要从事天然产物化学与利用研究; E-mail: chengx9013@163.com
基金资助:
四川省院省校合作项目(2019YFSY0002);中国林科院林产化学工业研究所研究团队建设创新工程项目(LHSXKQ2)

Oil Cell Morphology, Essential Oil Constituents and Antioxidant Activity of Cinnamomum longepaniculatum Leaves Harvested in Spring and Autumn

Xian CHENG¹(), Liangwu BI¹^,*(), Shengnan LI¹, Yuxiang CHEN¹, Zhendong ZHAO¹, Kailin MO²

1. Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab. for Biomass Chemical Utilization; Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grassland Administration; Key Lab. of Biomass Energy and Material, Jiangsu Province; Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing 210042, China
2. Sichuan Academy of Forestry Sciences, Chengdu 610081, China

Received:2020-11-10 Online:2021-02-28 Published:2021-03-03
Contact: Liangwu BI E-mail:chengx9013@163.com;biliangwu@126.com

摘要/Abstract

摘要：

首先利用组织透明法对春秋两季油樟叶中油细胞进行形态观察，统计油细胞密度和直径，利用扫描电镜（SEM）进一步观察油细胞的细胞壁结构；其次对春秋两季油樟叶进行油樟精油的提取，利用GC-MS鉴定油樟精油的化学成分，利用GC分析了1，8-桉叶素、α-松油醇和γ-松油烯3种主要成分的含量；最后对油樟精油的抗氧化活性进行检测。研究结果表明：春季和秋季油樟叶片的油细胞密度分别为（41±3）和（38±2）个/mm²，油细胞直径分别为（51.87±1.64）和（36.89±2.64）μm，细胞壁厚度分别为1.65和0.63 μm；春季和秋季油樟叶的精油提取得率分别为4.45%和3.09%（以干质量计），GC-MS表征鉴定出16个化合物，3种主成分的含量没有显著性差异；春秋两季的油樟精油总抗氧化能力分别为6.50和5.50 U/mL，达到Vc的40%，清除DPPH自由基的半数抑制质量浓度（IC₅₀）均为0.2 g/L。

关键词: 油樟, 油细胞, 精油, 抗氧化活性

Abstract:

The tissue transparency method was used to observe the morphology of oil cells in the leaves of Cinnamomum longepaniculatum(Gamble) N. Chao ex H. W. Li in spring and autumn, and the density and diameter of the oil cells were determined. Cell wall structure of the oil cells was observed with scanning electron microscopy. GC-MS was used to characterize the chemical composition of the essential oils extracted from the leaves of C. longepaniculatumin in spring and autumn.Eucalyptol, α-terpineol, and γ-terpinene content was analyzed by GC. Finally, antioxidant activity of the essential oils was evaluated. The density of oil cells of C. longepaniculatum leaves in spring and autumn was (41±3) and (38±2) per mm², the diameter was (51.87±1.64) and (36.89±2.64) μm, and the cell wall thickness was 1.65 and 0.63 μm, respectively. The extraction yields of essential oils from C. longepaniculatum leaves were 4.45% and 3.09%(based on dry weight) in spring and autumn, respectively. Sixteen types of chemical compounds were identified with GC-MS, and no significant difference was observed in the content of the three main components. The total antioxidant capacity of the essential oils from C. longepaniculatum leaves harvested in spring and autumn was 6.50 and 5.50 U/mL, respectively (40% of Vc), and the IC₅₀ value corresponding to scavenging of DPPH free radicals was 0.2 g/L.

Key words: Cinnamomum longepaniculatum(Gamble) N. Chao ex H. W. Li, oil cells, essential oil, antioxidant activity

中图分类号:

TQ35

程贤, 毕良武, 李胜男, 陈玉湘, 赵振东, 莫开林. 春秋两季油樟叶的油细胞形态、精油成分及其抗氧化活性研究[J]. 林产化学与工业, 2021, 41(1): 38-44.

Xian CHENG, Liangwu BI, Shengnan LI, Yuxiang CHEN, Zhendong ZHAO, Kailin MO. Oil Cell Morphology, Essential Oil Constituents and Antioxidant Activity of Cinnamomum longepaniculatum Leaves Harvested in Spring and Autumn[J]. Chemistry and Industry of Forest Products, 2021, 41(1): 38-44.

图/表 5

图1

图2

表1

表2

图3

参考文献 16

1	郄光发, 曹丽雯, 刘宏明, 等. 我国城市市树选择应用现状及区域分布特征[J]. 林业科学, 2019, 55 (10): 76- 87.
	QIE G F , CAO L W , LIU H M , et al. Current situation and regional distribution of city-trees in China[J]. Scientia Silvae Sinicae, 2019, 55 (10): 76- 87.
2	FABIANA L S , RAQUEL V S S , PAOLA C B , et al. Chemical composition of the brazilian native Cinnamomum stenophyllum(Meisn.) Vattimo-Gil essential oil by GC-qMS and GC×GC-TOFMS, and its cytotoxic activity[J]. Arabian Journal of Chemistry, 2020, 13 (4): 4926- 4935. doi: 10.1016/j.arabjc.2020.01.012
3	NETOPILOVA M , HOUDKOVA M , URBANOVA K , et al. In vitro antimicrobial combinatory effect of Cinnamomum cassia essential oil with 8-ydroxyquinoline against Staphylococcus aureus in liquid and vapour phase[J]. Journal of Applied Microbiology, 2020, 129 (4): 1- 10.
4	TANGJITJAROENKUN J , TANGCHITCHAROENKHUL R , YAHAYO W , et al. Chemical compositions of essential oils of Amomum verum and Cinnamomum parthenoxylon and their in vitro biologicalproperties[J]. Journal of Herbmed Pharmacology, 2020, 9 (3): 223- 231. doi: 10.34172/jhp.2020.29
5	胡文杰, 戴彩华, 周升团. 油樟叶精油馏分的主要成分、抑菌活性及其主要单体成分抑菌机理研究[J]. 安徽农学通报, 2019, 25 (15): 14- 19.
	HU W J , DAI C H , ZHOU S T . Research on major constituents, bacteriostatic effect and antibacterial mechanism of fractions from Cinnamomum longepaniculatum leaves essential oils and its major monomer components[J]. Anhui Agricultural Science Bulletin, 2019, 25 (15): 14- 19.
6	胡文杰, 罗辉, 戴彩华. 油樟叶的化学成分及其生物活性研究进展[J]. 中国粮油学报, 2019, 34 (11): 140- 146.
	HU W J , LUO H , DAI C H . Research progress on chemical constituents and biological activities of Cinnamomum longepaniculatum leaves[J]. Journal of the Chinese Cereals and Oils Association, 2019, 34 (11): 140- 146.
7	李嘉欣, 孟斌斌, 朱凯. 樟树叶精油组成分析及抗氧化活性研究[J]. 林产化学与工业, 2020, 40 (1): 84- 90.
	LI J X , MENG B B , ZHU K . Components and antioxidant activity of camphor leaves essential oil[J]. Chemistry and Industry of Forest Products, 2020, 40 (1): 84- 90.
8	王艺蓓, 孙莉寒, 武剑栋, 等. 基于近红外光谱和GC-MS法对油樟叶中挥发油的抗氧化研究和化学组成分析[J]. 植物学研究, 2018, 7 (3): 251- 259.
	WANG Y B , SUN L H , WU J D , et al. Antioxidant activity and chemical composition analysis of essential oil from Cinnamomum longepaniculatum by Nirs and GC-MS[J]. Bulein of Botanical Research, 2018, 7 (3): 251- 259.
9	史峻铭, 李婷婷, 宋诗政, 等. 低共熔溶剂微波辅助萃取油樟精油及抗氧化特性分析[J]. 植物学研究, 2019, 8 (3): 307- 318.
	SHI J M , LI T T , SONG S Z , et al. Study on the microwave-assisted extractionof essential oil from Cinnamomum longipaniculatum with deep eutectic solvents[J]. Bulein of Botanical Research, 2019, 8 (3): 307- 318.
10	杨素华, 安家成, 黎贵卿, 等. 不同月份柠檬醛型樟树叶精油变化规律及最佳采叶时间[J]. 广西林业科学, 2020, 49 (1): 22- 25.
	YANG S H , AN J C , LI G Q , et al. Variation of leaf essential oils of citral Cinnamomum camphora in different months and the best picking time[J]. Guangxi Forestry Science, 2020, 49 (1): 22- 25.
11	孙娅东. 芳樟叶油细胞形态特征研究[D]. 南昌: 江西农业大学, 2018.
	SUN Y D. Study on morphological characteristics of Cinnamomum camphora(L.) Presl leaf oil cells[D]. Nanchang: Jiangxi Agricultural University, 2018.
12	GUZMÁN J P S , NGUYEN K , HART S C . Simple methods to remove microbes from leaf surfaces[J]. Journal of Basic Microbiology, 2020, 60 (8): 1- 5.
13	李雪, 王宏伟, 郑东然, 等. 杜香叶多糖的提取及抗氧化和抗肿瘤活性研究[J]. 林产化学与工业, 2017, 37 (5): 133- 138.
	LI X , WANG H W , ZHENG D R , et al. Extraction and antioxidant, anti-proliferative activities of polysaccharide from Ledum palustre L.leaves[J]. Chemistry and Industry Forest Products, 2017, 37 (5): 133- 138.
14	胡居吾, 吴磊, 涂招秀, 等. 蔓三七叶中分离绿原酸和异绿原酸及其抗氧化活性研究[J]. 天然产物研究与开发, 2019, 31 (1): 38- 43.
	HU J W , WU L , TU Z X , et al. Extraction and antioxidant activity of chlorogenic acids and isochlorogenic acids from Gynura procumbens (Lour.) Merr[J]. Natural Product Research and Development, 2019, 31 (1): 38- 43.
15	QUEIROZ M I , MARONEZE M M , MANETTI A G S , et al. Enhanced single-cell oil production by cold shock in cyanobacterial cultures[J]. Ciência Rural, 2018, 48 (11): 1- 8.
16	初庆刚, 胡正海. 油樟油细胞和粘液细胞发育的超微结构[J]. 林业科学, 2001, 37 (4): 19- 25.
	CHU Q G , HU Z H . Developmental ultrastructure of oil and mucilage cells in Cinnamomum longepaniculatum[J]. Scientia Silvae Sinicae, 2001, 37 (4): 19- 25.

序号 No.	t_R/min	化合物 compound	分子式 fomula	特征离子碎片 characteristic fragments	相对峰面积relative area/%
序号 No.	t_R/min	化合物 compound	分子式 fomula	特征离子碎片 characteristic fragments	春季spring	秋季autumn
1	6.955	α-蒎烯α-pinene	C₁₀H₁₆	136/93	0.23	0.31
2	7.217	β-蒎烯β-pinene	C₁₀H₁₆	136/134	1.96	2.23
3	9.460	β-水芹烯β-phellandrene	C₁₀H₁₆	136/128	6.75	5.36
4	9.530	莰烯camphene	C₁₀H₁₆	136/119		0.05
5	12.623	邻伞花烃o-cymene	C₁₀H₁₄	134/105	0.13	0.29
6	13.415	对伞花烃p-cymene	C₁₀H₁₄	134/103	0.12	0.33
7	13.887	1, 8-桉叶素1, 8-cineole	C₁₀H₁₈O	154/139	51.59	57.25
8	19.995	罗勒烯ocimene	C₁₀H₁₆	136/121		0.05
9	31.724	侧柏烯thujene	C₁₀H₁₆	136/119	0.15	0.30
10	32.865	4-松油醇4-terpilenol	C₁₀H₁₈O	154/146	3.18	4.99
11	33.945	α-松油醇α-terpilenol	C₁₀H₁₈O	154/136	28.95	24.91
12	35.413	α-水芹烯α-phellandrene	C₁₀H₁₆	136/132	0.07	0.23
13	36.075	γ-松油烯γ-terpinene	C₁₀H₁₆	136/131	1.55	2.30
14	36.454	石竹烯caryophyllene	C₁₅H₂₄	204/79	1.94	0.79
15	36.659	甘香烯elixene	C₁₅H₂₄	204/93	0.82	0.37
16	36.887	大根香叶烯D germacrene D	C₁₅H₂₄	204/91	1.34	0.24

主要成分 main compounds	校正曲线 calibration curves	线性范围/(g·L^-1) linearity range	R²	质量浓度 mass concn./(g·L^-1)		P值 P value
主要成分 main compounds	校正曲线 calibration curves	线性范围/(g·L^-1) linearity range	R²	春季 spring	秋季 autumn	P值 P value
1, 8-桉叶素1, 8-cineole	y =169114x+16435	0.07~21.43	1	569.83±11.13	秋季 autumn	591.28±18.39	0.33
α-松油醇α-terpineol	y = 199028x+19880	0.07~21.23	0.9999	104.78±17.41	96.50±24.80	0.17
γ-松油烯γ-terpinene	y =201017x+19880	0.06~18.73	1	7.60±2.51	10.69±1.77	0.71

[1]	程晨, 司红燕, 王宗德, 廖圣良, 邹晨阳, 王鹏. O/W型山苍子精油微乳的制备及其性能研究[J]. 林产化学与工业, 2021, 41(1): 67-76.
[2]	程贤,毕良武,曾维星,赵振东,陈玉湘,张笮晦. 肉桂蒸油剩余物中活性成分的提取、纯化及其抗氧化活性研究[J]. 林产化学与工业, 2020, 40(2): 109-116.
[3]	李子江,刘慰,吴磊,王丹,司传领. 偃松松塔多糖的单糖组成分析及活性研究[J]. 林产化学与工业, 2020, 40(1): 37-44.
[4]	李嘉欣,孟斌斌,朱凯. 樟树叶精油组成分析及抗氧化活性研究[J]. 林产化学与工业, 2020, 40(1): 84-90.
[5]	王洁,路娇娇,杜涵,张萌,刘盼盼,陈小强. 五味子果肉和种子精油的化学成分与生物活性对比研究[J]. 林产化学与工业, 2019, 39(6): 59-67.
[6]	闫红秀, 敬雪敏, 刘香萍. 微波法提取兴安百里香精油及其主要组分活性研究[J]. 林产化学与工业, 2019, 39(5): 121-128.
[7]	刘义军,袁源,刘洋洋,张帆,李积华. 干燥方法对互叶白千层理化特性及精油品质的影响[J]. 林产化学与工业, 2019, 39(4): 77-82.
[8]	郑红富,廖圣良,范国荣,王宗德,陈尚钘,司红燕. 水蒸气蒸馏提取芳樟精油及其抑菌活性研究[J]. 林产化学与工业, 2019, 39(3): 108-114.
[9]	徐士超,曾小静,董欢欢,赵振东. 萜类植物源除草活性物开发及应用研究进展[J]. 林产化学与工业, 2019, 39(2): 1-8.
[10]	张慧芳,王叶,户维超,袁澍,袁明. 绵马贯众多酚的分离纯化及其抗氧化活性研究[J]. 林产化学与工业, 2019, 39(2): 81-88.
[11]	徐士超,董欢欢,曾小静,赵振东. 萜类植物源农药的筛选及活性研究进展[J]. 林产化学与工业, 2019, 39(1): 1-12.
[12]	孟斌斌, 朱凯. 杉木屑干馏及干馏产物组成的研究[J]. 林产化学与工业, 2018, 38(6): 88-94.
[13]	姜萍, 张茜, 赵雅洁, 陆婷婷, 王飞, 张力平. 巨尾桉树皮总多酚的提取分离及其生物活性研究[J]. 林产化学与工业, 2018, 38(5): 37-44.
[14]	周凌云, 鲁斌, 余小红, 张明芮, 华燕. 隐孔菌中倍半萜类化合物及其抗氧化活性研究[J]. 林产化学与工业, 2018, 38(1): 73-80.
[15]	陈虹霞, 康秀棠, 尤龙杰, 王成章, 叶建中. 缅甸大果紫檀精油的化学成分及其抑菌活性研究[J]. 林产化学与工业, 2017, 37(6): 65-72.

春秋两季油樟叶的油细胞形态、精油成分及其抗氧化活性研究

Oil Cell Morphology, Essential Oil Constituents and Antioxidant Activity of Cinnamomum longepaniculatum Leaves Harvested in Spring and Autumn

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 16

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

投稿指南

在线期刊

相关单位

联系我们