水热预处理竹材制备低聚木糖和单糖的研究

doi:10.3969/j.issn.0253-2417.2021.01.011

林产化学与工业 ›› 2021, Vol. 41 ›› Issue (1): 77-84.doi: 10.3969/j.issn.0253-2417.2021.01.011

水热预处理竹材制备低聚木糖和单糖的研究

詹云妮¹, 黄晨¹^,², 郝昕², 王梓萌², 邓拥军¹^,², 房桂干¹^,²^,*()

1. 中国林业科学研究院林产化学工业研究所; 生物质化学利用国家工程实验室; 国家林业和草原局林产化学工程重点实验室; 江苏省生物质能源与材料重点实验室, 江苏南京 210042
2. 南京林业大学江苏省林业资源高效加工利用协同创新中心, 江苏南京 210037

收稿日期:2020-07-15 出版日期:2021-02-28 发布日期:2021-03-03
通讯作者: 房桂干 E-mail:fangguigan@icifp.cn
作者简介:房桂干, 研究员, 博士生导师, 研究领域为木质纤维资源的综合利用; E-mail: fangguigan@icifp.cn
詹云妮(1997-), 女, 河南信阳人, 硕士生, 研究方向为木质纤维原料的生物化学加工
基金资助:
江苏省生物质能源与材料重点实验室基本科研业务费项目(JSBEM-S-202004);国家自然科学基金青年项目(32001273);中国博士后科学基金面上项目(2020M670526)

Preparation of Xylooligosaccharides and Monosaccharides from Bamboo Chips by Liquid Hot Water Pretreatment

Yunni ZHAN¹, Chen HUANG¹^,², Xin HAO², Zimeng WANG², Yongjun DENG¹^,², Guigan FANG¹^,²^,*()

1. Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab. for Biomass Chemical Utilization; Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grassland Administration; Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China
2. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China

Received:2020-07-15 Online:2021-02-28 Published:2021-03-03
Contact: Guigan FANG E-mail:fangguigan@icifp.cn

摘要/Abstract

摘要：

以贵州慈竹为研究对象，对其进行水热预处理（LHWP），研究不同预处理强度系数对竹材化学组分、酶水解性能及低聚木糖（XOS）浓度的影响。利用X射线衍射（XRD）和傅里叶转换红外线光谱分析仪（FT-IR）分析预处理前后物料的物理和化学结构变化。研究结果表明：水热预处理过程中竹材的半纤维素含量显著降低，而纤维素及木质素的含量则有所增加；水热预处理能够显著提升竹材的酶解效率，在预处理强度系数为4.50时，预处理竹材的酶水解性能最高，葡聚糖和木聚糖酶水解得率分别为79.0%和92.0%；而XOS质量浓度则在强度系数为3.96时，达到最大值8.7 g/L（得率55.3%），继续提高预处理强度，XOS质量浓度降低，在强度系数为4.50时，体系中仅检测到0.5 g/L（得率3.1%）XOS。

关键词: 水热预处理, 竹材, 酶水解, 低聚木糖

Abstract:

Effects of liquid hot water pretreatment(LHWP) on the chemical composition, enzymatic digestibility, and xylooligosaccharides(XOS)concentration were investigated in Bambusa emeiensis. In addition, the physical and chemical structural variationsof thesamples before and after the pretreatment were studied using a X-ray diffraction(XRD)spectrometer and Fourier transform infrared spectroscopy(FT-IR). Results indicated that the xylan content was significantly decreased after LHWP, while the contents of glucan and lignin increasing accordingly. LHWP improved the enzymatic digestibility of the bamboo chips feedstock, and the enzymatic hydrolysis yield reached the maximum of 79.0%(glucan) and 92.0%(xylan) at a pretreatment intensity coefficiency of 4.50. However, the XOS concentration reached the maximum value(8.7 g/L)at an intensity coefficiency of 3.96(relative mass percentage of 55.3%), and decreased thereafter as the pretreatment strength was further increased. Finally only 0.5 g/L(relative mass percentage of 3.1%)XOS was obtained in the pretreatment prehydrolysate at an intensity coefficiency of 4.50.

Key words: liquid hot water pretreatment, bambaoo, enzymatic hydrolysis, xylooligosaccharides

中图分类号:

TQ35

詹云妮, 黄晨, 郝昕, 王梓萌, 邓拥军, 房桂干. 水热预处理竹材制备低聚木糖和单糖的研究[J]. 林产化学与工业, 2021, 41(1): 77-84.

Yunni ZHAN, Chen HUANG, Xin HAO, Zimeng WANG, Yongjun DENG, Guigan FANG. Preparation of Xylooligosaccharides and Monosaccharides from Bamboo Chips by Liquid Hot Water Pretreatment[J]. Chemistry and Industry of Forest Products, 2021, 41(1): 77-84.

图/表 8

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预处理强度系数(lgR₀) pretreatment intensity coefficiency	物料得率/% solid recovery	葡聚糖/% glucan	木聚糖/% xylan	木质素/% lignin
0	100.0	44.5	15.8	29.1
3.66	76.5	49.4	11.3	31.0
3.96	69.1	54.5	7.4	29.7
4.13	68.0	56.6	5.0	31.5
4.26	59.6	58.8	4.6	31.5
4.36	56.1	59.7	4.2	31.3
4.50	41.7	55.5	3.5	34.9

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水热预处理竹材制备低聚木糖和单糖的研究

Preparation of Xylooligosaccharides and Monosaccharides from Bamboo Chips by Liquid Hot Water Pretreatment

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