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林产化学与工业

林产化学与工业 ›› 2013, Vol. 33 ›› Issue (6): 85-90.doi: 10.3969/j.issn.0253-2417.2013.06.016

• 1研究报告 • 上一篇    下一篇

玄参总三萜提取物制备及其抗氧化生物活性研究

陈莉华, 吴玲, 李林芝, 贺玲芝   

  1. 吉首大学 化学化工学院, 湖南 吉首 416000
  • 收稿日期:2012-11-22 修回日期:1900-01-01 出版日期:2013-12-30 发布日期:2013-12-30

Preparation and Antioxidation Biological Activity of Total Triterpenoids Extracts from Scrophularia ningponesis

CHEN Li-hua, WU Ling, LI Lin-zhi, HE Ling-zhi   

  1. College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000，China
  • Received:2012-11-22 Revised:1900-01-01 Online:2013-12-30 Published:2013-12-30

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摘要： 通过单因素和正交试验研究超声波辅助乙醇提取玄参总三萜，讨论了料液比、乙醇体积分数、超声波时间、温度等因素对提取效果的影响，用大孔树脂进行纯化并用齐墩果酸对照品测定含量，考察了纯化后的总三萜提取物对羟基自由基（·OH）的清除效果，以没食子酸和Vc为对照品，对总三萜提取物抑制油脂氧化进行评估。结果表明：在超声波功率 250 W、 70 ℃ 下，选择 70% 乙醇为提取剂，料液比为1：30（g：mL），超声波提取 40 min 后，2.0 g 玄参原料经过2次提取，5次平行实验总三萜得率平均值为 2.56%，纯化后齐墩果酸质量分数为 88.50%。玄参总三萜提取物对羟基自由基的清除率 50% 时即IC50所对应的总三萜质量浓度为 38 mg/L，当在 40 ℃ 烘箱中强化放置 360 min 后，总三萜质量浓度为 12 mg/L 的提取物对植物油的保护率为 20.5%，对动物油的保护率为 29.0%，相同条件下玄参总三萜提取物对动物油的抗氧化效果强于没食子酸和Vc，对植物油的抗氧化效果与没食子酸和Vc相当。

关键词: 玄参, 总三萜提取物, 羟基自由基, 油脂, 抗氧化生物活性

Abstract: Total triterpenoids was extracted from Scrophularia ningponesis Hemsl. with ethanol and ultrasonic-assisted. The influence factors such as material-to-liquid ratio(g:mL), ethanol volume fraction, ultrasonic extracting time and temperature were investigated based on single factor and orthogonal test. The total triterpenoids extract was purified by macro-porous resin and analyzed with oleanic acid. Research on hydroxyl radicals scavenging of purified total triterpenoids extracts was carried out. The antioxidation to oils and fats of purified total triterpenoids extracts were evaluated and compared with gallic acid and Vc. The results showed that the optimal extracting conditions were obtained as follows: 70% ethanol, 1:30(g:mL) material-to-liquid ratio, 40 min ultrasonic extracting time at 250 W and 70 ℃,twice extraction. Under above conditions, the extracts yield was 2.56% and the content of oleanic acid in purified total triterpenoids extracts was 88.50%. The purified total triterpenoids extracts exhibited excellent ability to remove ·OH and the oleanic acid concentration of IC50 to ·OH was 12 mg/L. When antioxidation test was carried out at the condition of being kept in oven at 40 ℃ for 360 min, the protection rate was 20.5% to oil and 29.0% to fat,respectively. The antioxidating effect of purified total triterpenoids extracts was better than that of gallic acid and Vc to oils and almost the same as gallic acid and Vc to fats, respectively.

Key words: Scrophularia ningponesis Hemsl., total triterpenoids extracts, hydroxyl radicals, oils and fats, antioxidation biological activity

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