介孔氧化钛与海藻酸钠固定化木聚糖酶的对比研究

doi:10.3969/j.issn.0253-2417.2013.01.012

›› 2013, Vol. 33 ›› Issue (1): 64-68.doi: 10.3969/j.issn.0253-2417.2013.01.012

• 1研究报告 • Previous Articles Next Articles

Comparison of Immobilized Xylanase Using Mesoporous Titania and Sodium Alginate as Carriers

SHAN Zong-xing¹, WEI Ce², LI Xin^1,3, SONG Xiang-yang^1,3, ZHANG Yang¹, ZHANG Wei-wei¹, XU Chen-chen¹

1. The College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China;2. Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211800, China;3. Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing 210037, China

Received:2012-09-24 Revised:1900-01-01 Online:2013-02-28 Published:2013-02-28

Abstract

Abstract: In order to improve the stability of xylanase in biocatalysi, mesoporous titania (M-TiO₂) and sodium alginate were used to immobilize xylanase. The properties of immobilized xylanase were also investigated in this study. The optimum immobilization by mesoporous titiania was obtained as 20.36 IU/g xylanase was immobilized at 65 ℃ for 0.5 h under pH 6.0. The maximal immobilized rate is 93.37%.In contrast, the xylanase was immobilized when 35 g/L sodium alginate was selected to handle the enzyme at 65 ℃ for 2 h under pH 6.0 environment in the presence of 20 g/L CaCl₂. Maximal immobilized rate is 28.77%.In regard of the free and immobilized xylanase on sodium alginate, immobilized xylanase by mesoporous titania has better thermostability and pH stability.

Key words: xylanase, immobilization, mesoporous titania, sodium alginate, stability

CLC Number:

TQ35
Q814

SHAN Zong-xing;WEI Ce;LI Xin;SONG Xiang-yang;ZHANG Yang;ZHANG Wei-wei;XU Chen-chen. Comparison of Immobilized Xylanase Using Mesoporous Titania and Sodium Alginate as Carriers[J]. , 2013, 33(1): 64-68.

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Comparison of Immobilized Xylanase Using Mesoporous Titania and Sodium Alginate as Carriers

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