Welcome to Chemistry and Industry of Forest Products,

›› 2013, Vol. 33 ›› Issue (4): 83-88.doi: 10.3969/j.issn.0253-2417.2013.04.016

• 1研究报告 • Previous Articles     Next Articles

Purification, Degradation and Scavenging Activities on DPPH Free Radical of Proanthocyanidins from Black Wattle Bark

ZHAO Zhi-jie, XIONG Jia, WANG Fei   

  1. College of Chemistry Engineering, Nanjing Forestry University; Jiangsu Key Lab of Biomass-based green Fuels and Chemicals, Nanjing 210037, China
  • Received:2012-11-12 Revised:1900-01-01 Online:2013-08-30 Published:2013-08-30

Abstract: Proanthocyanidins were extracted from black wattle bark by the ultrasonic technology and purified on AB-8 resin. The oligomers were extracted with ethyl acetate and polymers in water were degraded by H2SO3. Finally, the scavenging activities on 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH·) of each fraction were determined. The results showed that the mass fraction of proanthocyanidins in crude extraction increased from 36.2% to 50.4% by purification on AB-8 resin. The recovery was 90.3%. The proanthocyanidins were degraded by H2SO3 significantly and the average degree of polymerization drop to 6.25 from 8.11 after degradation. It was found that the proanthocyanidins after degradation had higher DPPH scavenging activity and EC50reduced from 2.25% to 1.88%.

Key words: proanthocyanidins, purification, degradation, DPPH free radical

CLC Number: