Abstract: Biological treatment was used to remove glucose and galactose in this paper to purify the hemicellulose liquid sugars. Single factor and central composite experiments were used to optimize the medium components in the biological treatment. It was seen that the optimal results were obtained when 5.7 g/L (NH₄)₂SO₄, 2.0 g/L KH₂PO₄ and 2.4 g/L yeast were applied. The removal of glucose and galactose were 100% and 67%, and the purity of xylose increased from 47% to 60% after the optimal biological treatment. Acclimatization of strain used in the biotreatment of the liquor was also conducted to handle the high sugar consistency liquor which was diluted 6 times.

Key words: hemicellulose hydrolysis liquor, biotreatment, purification, xylose, acclimatization