米根霉无载体固定化发酵产L-乳酸

doi:10.3969/j.issn.0253-2417.2014.05.021

Abstract

Abstract: The main factors affecting self-immobilization of Rhizopus oryzae NLX-M-1 for L-lactic acid production were studied in present study. The optimum fermentation conditions utilizing wood fiber-based glucose for R.oryzae self-immobilization were obtained. The optimal results were achieved as 100 g/L initial glucose, 2 g/L (NH₄)₂SO₄, 2 %inoculum size (value fraction), 30 g/L CaCO₃, 0.1 g/L KH₂PO₄, 0.25 g/L MgSO₄·7H₂O and 0.1 g/L ZnSO₄·7H₂O. Under this optimal condition, R.oryzae formed pellets with an average diameter of about 1 mm during the fermentation, the production of L-lactic acid was 76.6 g/L and the conversion rate was 81.6 %. R.oryzae in acid explosive residue hydrolyzate containing 60 g/L glucose became 1.2 mm pellets, and produced 36.4 g/L L-lactic acid with a conversion rate of 63.5 %.

Key words: Rhizopus oryzae, self-immobilize, L-lactic acid, corn stover, acid explosive residue

CLC Number:

TQ35

CHEN Xiao-pei, ZHANG Li, GU Xi-mei, LI Xin, YONG Qiang, YU Shi-yuan. Production of L-Lactic Acid by Self-immobilized Rhizopus oryzae[J]. Chemistry and Industry of Forest Products, 2014, 34(5): 127-132.

References

[1] 汪多仁.乳酸的生产与应用[J] .化学工业与工程技术,1999,20(1):27-29.
[2] 郭英,付涛.酸化剂-乳酸在饲料添加剂中的应用[J] .河北化工,2011,34(6):62-63,71.
[3] 白冬梅,赵学明,李鑫钢,等.米根霉发酵生产L(+)-乳酸研究进展[J] .现代化工,2002,22(6):9-13.
[4] HAMAMCI H,RYU D D Y.Production of L(+)-lactic acid using immobilized Rhizopus oryzae reactor performance based on kinetic model and simulation[J] .Applied Biochemistry and Biotechnology,1994,44(2):125-133.
[5] 蒋雪薇,罗晓明,盛灿梅,等.米根霉L-乳酸发酵菌丝球形成条件的研究[J] .食品科学,2010,31(15):216-220.
[6] DU J X,CAO N J,GONG C S,et al.Biotechnology for Fuels and Chemicals[M] .New York:Humarn Press,1998:323-329.
[7] 白凤武.无载体固定化细胞的研究进展[J] .生物工程进展,2000,20(2):32-36.
[8] ZHANG Zhan-ying,JIN Bo,KELLY J M.Production of lactic acid from renewable materials by Rhizopus fungi[J] .Biochemical Engineering Journal,2007,35(3):251-263.
[9] 姜绍通,郑志,朱羽,等.无载体固定化米根霉重复间歇发酵生产L-乳酸[J] .生物工程学报,2008,24(10):1729-1733.
[10] BAI D M,JIA M Z,ZHAO X M,et al.L(+)-lactic acid production by pellet-form Rhizopus oryzae R1021 in a stirred tank fermentor[J] .Chemical Engineering Science,2003,58(3):785-791.
[11] SLUITER A,HAMES B,RUIZ R,et al.Biomass Laboratory Analytical Procedure (LAP)[S] .2012-03-08.
[12] 马瑞,欧阳嘉,李鑫,等.高效液相色谱法同时测定生物质乳酸发酵液中有机酸及糖类化合物[J] .色谱,2012,30(1):62-66.
[13] 李鑫,马瑞,赵晗璐,等.米根霉利用纯糖和不同预处理玉米秸秆酶解糖生产L-乳酸[J] .生物加工过程,2010,8(6):1-5.
[14] 姜绍通,吴学凤,潘丽军,等.米根霉菌丝球半连续发酵产乳酸的工艺研究[J] .农业机械学报,2009,40(11):150-155.
[15] 朱羽.无载体固定化米根霉发酵L-乳酸研究[D] .合肥:合肥工业大学硕士学位论文,2007.
[16] 蒲洋,丁涓,李静,等.不同中和剂对L-乳酸发酵的影响[J] .安徽农业科学, 2009,37(11):4885-4886,4888.
[17] 杜威.米根霉发酵生产L-乳酸的形态研究及其对发酵的影响[D] .合肥:合肥工业大学硕士学位论文,2010.
[18] 姜绍通,吴学凤,刘靖,等.无载体固定化米根霉半连续发酵产L-乳酸工艺研究[J] .食品科学,2009,30(9):124-128.
[19] FOSTER J W.Chemical Activities of Fungi[M] .New York:Academic Press,1949.
[20] ZHOU Y,DU J,TSAO G T.Mycelial pellet formation by Rhizopus oryzae ATCC 20344[J] .Applied Biochemistry and Biotechnology,2000,84(1/9):779-789.
[21] 乔长晟,汤凤霞,白雪娟,等.利用纤维素类物质——玉米芯生产L-乳酸的研究[J] .中国食品学报,2006,6(4):101-105.
[22] TAI C,LI S,XU Q,et al.Chitosan production from hemicellulose hydrolysate of corn straw:Impact of degradation products on Rhizopus oryzae growth and chitosan fermentation[J] .Letters in Applied Microbiology,2010,51(3):278-284.

Production of L-Lactic Acid by Self-immobilized Rhizopus oryzae

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LAI Chen-huan, YANG Lei, GAO Zi-qi, YU Shi-yuan, YONG Qiang. Evaluation of the Factors Contributing to Declining Enzymatic Hydrolysis Rates of Steam Pretreated Corn Stover [J]. Chemistry and Industry of Forest Products, 2016, 36(4): 7-13.
[2]	LI Cheng, WANG Li, LI Yong-fu, WANG Ren, CHEN Zheng-xing. Effects of Ozone Pretreatment on the Enzymatic Hydrolysis Efficiency of Corn Stover [J]. Chemistry and Industry of Forest Products, 2015, 35(6): 58-62.
[3]	ZHU Yuan-yuan, YANG Jin-long, ZHU Jun-jun, ZHANG Lu, XU Yong, YONG Qiang, YU Shi-yuan. Ethanol Production from Dilute Sulfuric Acid-calciumHydroxide Co-pretreated Corn Stover [J]. Chemistry and Industry of Forest Products, 2015, 35(6): 89-95.
[4]	YANG De-liang, CHU Qiu-lu, LAI Chen-huan, XIE Yi-hui, LI Xin, YU Shi-yuan, YONG Qiang. Semi-simultaneous/Simultaneous Saccharification and Fermentation of Corn Stover [J]. Chemistry and Industry of Forest Products, 2015, 35(3): 55-59.
[5]	JIANG Fa-xian, XU Yong, ZHU Jun-jun, YONG Qiang, YU Shi-yuan. Degradation of Four Non-lignin Components in Corn Stover by Pretreatment of Dilute Sulfuric Acid [J]. Chemistry and Industry of Forest Products, 2015, 35(2): 38-46.
[6]	WEI Bing, CHEN Fan-geng, LI Zhi-hao, CHENG Yuan-chao. Liquefaction of Fermentation Residue of Corn Stover in Polyhydric Alcohols [J]. Chemistry and Industry of Forest Products, 2014, 34(5): 53-59.
[7]	ZHU Jun-jun, YANG Jin-long, ZHANG Ling-ling, HUANG Xin, XU Yong, YONG Qiang, YU Shi-yuan. Detoxification and Ethanol Fermentation of the Acid-catalysed Steam-exploded Corn Stover Pre-hydrolyzate [J]. Chemistry and Industry of Forest Products, 2014, 34(5): 60-66.
[8]	ZHU Jun-jun, ZHU Yuan-yuan, CHENG Ting, XU Yong, YONG Qiang, YU Shi-yuan. Effects of Hydrolyzate of Corn Stover Acetyl Groups on Ethanol Fermentation and Its Removal Strategy [J]. Chemistry and Industry of Forest Products, 2014, 34(1): 1-7.
[9]	CHU Qiu-lu;YANG De-liang;HUANG Yang;YU Shi-yuan;YONG Qiang. Ethanol Production from Detoxified Acid Steam Exploded Corn Stover by Washing [J]. , 2013, 33(6): 69-75.
[10]	CHU Qiu-lu;YANG De-liang;MA Bin;YONG Qiang. Integrated Process of Ethanol Production from Corn Stover [J]. , 2013, 33(5): 20-24.
[11]	OUYANG Jia;LI Xin;CHEN Mu;LIAN Zhi-na. Enhancement Effect of Exogenous Additives on Enzymatic Conversion of Moderate Temperature Alkali-treated Corn Stover Residue [J]. , 2012, 32(4): 5-9.
[12]	YAO Lan;ZHAO Jian;XIE Yi-min;YANG Hai-tao;YANG Wu-feng;QU Yin-bo. Mechanism of Diluted Acid Pretreatment to Improve Enzymatic Hydrolysis of Corn Stover [J]. , 2012, 32(4): 87-92.
[13]	ZHU Jun-jun;CHEN Shang-xing;YONG Qiang;XU Yong;CHEN Mu;YU Shi-yuan. Biorefinery of Corn Stover for Fuel Ethanol [J]. , 2011, 31(6): 35-40.
[14]	QU Liang;XU Yong;ZHANG Hong-man;HUANG He;YING Han-jie. Detoxification of Steam-exploded Lignocellulosic Material by Ethanol Extraction [J]. , 2010, 30(1): 22-26.
[15]	FANG Hao;SONG Xiang-yang;ZHAO Chen;CHANG Zheng;CHU Jie;YONG Qiang. Cellulase Production from Mixed Fermentation of Trichoderma reesei RUT C-30 and Apsergillus niger NL02 [J]. , 2009, 29(6): 15-19.