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Chemistry and Industry of Forest Products ›› 2014, Vol. 34 ›› Issue (5): 145-151.doi: 10.3969/j.issn.0253-2417.2014.05.024

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Ultrasonic Extraction and Antioxidant Activity of Polyphenol from Synsepalum dulcificum Leaf

LU Sheng-lou, FU Jia-zhen, LIU Hong, FU Chun-min, LIN You-ming, WANG Xiao-he   

  1. College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou Key Laboratory of Research and Development on Topical and Special Medicine and Edible Plant, Haikou 571127, China
  • Received:2013-03-12 Revised:2014-06-18 Online:2014-10-25 Published:2015-08-18
  • Contact: 刘红(1967-),女,山东诸城人,教授,博士,硕士生导师,研究领域为天然产物分离新技术;E-mail:lhyd123@sohu.com E-mail:lhyd123@sohu.com

Abstract: The purpose of this paper was to determine the optimum technology and conditions to extract polyphenol from the leaves of Synsepalum dulcificum. Based on the results of single factor experiments, by using the response surface method the S. dulcificum leaves polyphenols mathematical model was established. Meanwhile, the antioxidant activity of the extracted polyphenol was evaluated. The best technical conditions to extract polyphenol from S. dulcificum leaf were obtained as follows:the concentration of acetone solution 58%, ultrasonic time 72 min and extraction temperature 48 ℃, when the S.dulciticum leaf were 1.0 g and liquid-solid ratio was 30:1(mL:g). The predicted polyphenol extraction yield was 6.82%. Under the optimized condition, the experimental yield was 6.84 % which agreed with its predicted yield. The in vitro antioxidant assay results showed that S. dulcificum leaf polyphenol exhibited a dose-dependent scavenging activity against 2,2'-azinobis (3-ethyl- benzothiazoline-6-sulfonic acid ammonium salt(ABTS) radical, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and ·OH radical. Its hemi-inhibiting concentrations (IC50) were 51.81,13.40 and 28.91 mg/L, respectively. Which suggested that S. dulcificum leaf had high antioxidant activity.

Key words: Synsepalum dulcificum leaf, polyphenol, ultrasound extraction, antioxidant activity

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