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Chemistry and Industry of Forest Products ›› 2015, Vol. 35 ›› Issue (2): 47-54.doi: 10.3969/j.issn.0253-2417.2015.02.007

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Phenolic Extracts from Rhus verniciflua Stokes Woods by Vacuum Extraction and Their Anti-tyrosinase Activities

CHEN Hong-xia1,2, WANG Cheng-zhang1,2, YE Jian-zhong2, ZHOU Hao1,2, ZHANG Yu-si2, TAO Ran2   

  1. 1. Research Institute of Forestry New Technology, CAF, Beijing 100091, China;
    2. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab.for Biomass Chemical Utilization;Key and Open Lab. of Forest Chemical Engineering, SFA;Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China
  • Received:2014-09-03 Online:2015-04-25 Published:2015-04-24

Abstract: The polyphenols were extracted from Rhus verniciflua Stokes by vacuum extraction, and the anti-tyrosinase activities were studied. The optimal conditions were investigated through single factor and response surface method. The optimal parameters were vacuum degree 0.082 MPa, ethanol volume fraction 83%, extraction time 1.5 h, liquid-solid ratio 20:1(mL:g), and extracted twice. At these conditions, the yield of polyphenol was 2.193%. Though the extraction, the ethyl acetate extract with 53.43% of phenolic was obtained and the inhibitory rate of tyrosinase was 63.4%. The ethyl acetate extract concentration leading to 50% inhibitory rate was 308 mg/L. The ethyl acetate extract could extend the lag time of tyrosine monophenolase, and 500 mg/L of extract could decrease the velocity from 29 μmol/(L·min) to 8 μmol/(L·min). The ethyl acetate extract functioned as a hybrid inhibition of tyrosine diphenolase by Lineweaver-Burk plots.

Key words: Rhus verniciflua Stokes, polyphenol, vacuum extraction, tyrosinase, inhibition rate

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