超临界CO2提取油橄榄叶中橄榄苦苷及其结构鉴定

doi:10.3969/j.issn.0253-2417.2015.02.011

Abstract

Abstract: The Olea europaea leaves were extracted by supercritical carbon dioxide extraction technology, then the extract was analyzed by silica gel column chromatography. A compound was isolated from the extract with content of 96% which was identified as oleuropein by HPLC-MS, ¹H NMR, and IR. The HPLC analysis was carried out with column C₁₈ and MeOH-water 40:60 (volume ratio) as mobile phase at flow rate 0.5 mL/min, and monitored at 254 nm. By single factor and orthogonal experiments, optimal extraction conditions were obtained as follows: extraction pressure 25 MPa, extraction temperature 45 ℃, separation temperature 45 ℃,extraction time 4 h, and CO₂ flow rate 30 kg/h. At the optimal extraction conditions, the yield of oleuropein was 0.435%.

Key words: Olea europaea leaves, supercritical carbon dioxide extraction, oleuropein, extraction technology, chemical structure

CLC Number:

TQ35

GUO Yu-yu, WANG Ling, LUO Jin-yue, SHAO Shuang-xi, WANG Cheng-zhang, LIU Zu-guang. Extraction and Structure Identification of Oleuropein from Olea europaea Leaves by Supercritical Carbon Dioxide Extraction Technology[J]. Chemistry and Industry of Forest Products, 2015, 35(2): 73-78.

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Extraction and Structure Identification of Oleuropein from Olea europaea Leaves by Supercritical Carbon Dioxide Extraction Technology

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