Abstract: To screen high-yield strains which could produce paclitaxel and 10-deacetylbaccation Ⅲ(10-DAB) from T.chinensis var.mairei mycorrhizal fungus,21 strains were separated, obtained and carried on liquid fermentation culture.The detection results of TLC,HPLC,and LC-MS show that 10 strains among those possess the ability to produce paclitaxel and taxane,and 10-DAB contents of 4 strains are 260.05, 92.88,393.27 and 70.52 μg/L,respectively.The paclitaxel contents of 6 strains are 434.94,1 242.19,200.65,360.77,317.90 and 29.82 μg/L among these 10 strains.These results indicate that the mycorrhizal fungus has the ability to synthesize paxlitaxel and taxanes, but each of the synthesized compounds have the difference. It is the different kinds of mycorrhizal that may participate in the taxol biosynthesis, and high contents of taxol result form collaborative actions. And there is the paclitaxel potential to produce in fermentation culture of the high-yield strains.

Key words: Taxus chinensis var.mairei, mycorrhizal fungus, paclitaxel, 10-deacetylbaccatin Ⅲ, liquid fermentation