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Chemistry and Industry of Forest Products ›› 2015, Vol. 35 ›› Issue (5): 10-14.doi: 10.3969/j.issn.0253-2417.2015.05.003

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HPLC and MALDI-TOF-MS Analysis of Hydrolysates of Xylan

LI Hai-long1, LI Cheng-guo1, QIN Guang-hui1, LIU Jian1, GAN Li-hui1, XIE Ru-sheng2, LONG Min-nan1   

  1. 1. College of Energy, Xiamen University, Xiamen 361102, China;
    2. Department of Pharmacy, Fujian Vocational College of Bio-engineering, Fuzhou 350002, China
  • Received:2014-09-25 Online:2015-10-25 Published:2015-10-24

Abstract: The hydrolysis end products of beechwood xylan, which were released by sulfuric acid or enzymes and then labeled at their reducing ends with 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization, were analyzed by HPLC assisted with MALDI-TOF-MS. Some of the hydrolysates, which were lack of related commercial available standard substances, were determined. It was found that the xylose and 4-O-methyl-glucuronic acid-xylose (B2) were the main products with minor amounts of 4-O-methylglucuronic acid (B1) in the hydrolysates of beechwood xylan by sulfuric acid. Recombinant endo-β-1,4-xylanase AnXyn10C released xylose, xylobiose, and 4-O-methyl-glucuronic acid-xylotriose (B3) as the main hydrolysates from beechwood xylan, whereas recombinant endo-β-1,4-xylanase HoXyn11A released xylose, xylobiose, xylotriose, 4-O-methyl-glucuronic acid-xylotetrose (B4) and -xylopentaose (B5), and aldohexaouronic acid. These results revealed that HPLC assisted with MALDI-TOF-MS based on PMP derivatization was a very useful and robust method for the determination of products in hydrolysis of xylan.

Key words: 1-phenyl-3-methyl-5-pyrazolone, xylo-oligosaccharides, 4-O-methylglucuronic acid, MALDI-TOF-MS, HPLC

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