迷迭香叶中鼠尾草酸的分离纯化

doi:10.3969/j.issn.0253-2417.2015.05.017

Abstract

Abstract: Using the composite method of the following three steps to get carnosic acid from rosemary leaves, the purity of carnosic acid was 21.06% by ultrasound-assisted ethanol extraction. The purity of carnosic acid was 79.62% by ultrasonic-assisted n-hexane extraction and pH adjustment. The optimization conditions of ultrasonic-assisted n-hexane extraction were liquid/solid ratio 20:1(mL:g), ultrasonic frequency 28 kHz, ultrasonic time 40 min, ultrasonic power 140 W. Under these conditions, the yield of carnosic acid was 2.0%. The optimization conditions of silica gel column chromatography were particle sizes of silica gel 48-75 μm, the volume ratio of eluent ethyl acetate and petroleum ether 4:7, the flow rate 6 mL/min and 0.3 g solid loaded. The recovery rate of carnosic acid was 54.12%. The purity of carnosic acid was 95.18% by this method.

Key words: carnosic acid, ultrasound-assisted extraction, purification, column chromatography

CLC Number:

TQ35

XIA Tian-juan, BI Liang-wu, ZHAO Zhen-dong, CHENG Xian, XING Ya-li. Separation and Purification of Carnosic Acid from Rosemary Leaves[J]. Chemistry and Industry of Forest Products, 2015, 35(5): 105-110.

References

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Separation and Purification of Carnosic Acid from Rosemary Leaves

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