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Chemistry and Industry of Forest Products ›› 2015, Vol. 35 ›› Issue (5): 116-122.doi: 10.3969/j.issn.0253-2417.2015.05.019

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Chemical Constituents Extracted from Leaves of Prunus cerasifera Ehrh.cv. Atropurpurea Jacg. and Their Antioxidant Activities in vitro

WEI Qiang1, JI Xiao-ying1, LONG Xian-shun1, LI Qian-rong2, YIN Hao2   

  1. 1. Pharmacy School, Anhui XinHua University, Hefei 230088, China;
    2. Hefei National Laboratory for Physical Sciences at Micro Scale, University of Science and Technology of China, Hefei 230026, China
  • Received:2014-09-02 Online:2015-10-25 Published:2015-10-24

Abstract: Ethanol extracts from the leaves of Prunus cerasifera Ehrh.cv.Atropurpurea Jacg. were separated and purified by column chromatography over silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified by spectral methods including 1H, 13C NMR. Seventeen compounds were isolated and identified as β-sitosterol (1), stigmasterol (2), daucosterol (3), lupeol (4), ursolic acid (5), arjunolic acid (6), Niga-ichigoside F1 (7), apigenin (8), kaempferol (9), kaempferol-3-O-β-D-glucoside (10), quercetin (11), quercetin-4'-O-β-D- glucoside (12), hyperin (13), luteolin (14), luteolin-4'-O-β-D-glucoside (15), rutin (16), gallic acid (17). All compounds were isolated from the leaf for the first time. Compounds 11, 14 and 17 showed stronger antioxidant activities by DPPH scavenging activity with IC50 inhibition rates of 32.18, 34.65 and 34.12 mmol/L, respectively, compared with the Vc IC50 inhibition rate of 55.22 mmol/L.

Key words: Prunus cerasifera Ehrh.cv.Atropurpurea Jacg., chemical constituents, antioxidant activities

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