利用基因组DNA诱变技术选育高抑制物耐受性工业酵母菌

doi:10.3969/j.issn.0253-2417.2015.06.018

Abstract

Abstract: The recombinant Saccharomyces cerevisiae HN-1-24 was obtained by combination of chemical mutagenesis and genomic DNA mutagenesis-based genetic recombination methods to improve the inhibitor tolerance and fermentation performance.The recombinant strain HN-1-24 exhibited a high conversation rate of sugar to ethanol and ethanol production under a condition with 7 g/L acetic acid and 1 g/L furfural.Thus 38 g/L of ethanol could be obtained from 100 g/L of glucose.When ethanol fermentation of cellulose hydrolyzate culture was performed, the fermentation time of the recombinant strain was decreased by 6 h and the final ethanol concentration was enhanced by 8.8% as compared with the original strain.The results of this study provided a basis for the large-scale production of fuel ethanol by using lignocellulose.

Key words: Saccharomyces cerevisiae, ethanol, chemical mutagenesis, genetic recombination, inhibitor

CLC Number:

TQ35
Q813

CHEN Liang, XU Long-long, YAN De-ran, DU Feng-guang, HUI Feng-li. Breeding of Robust Inhibitor-tolerant Industrial Saccharomyces cerevisiae Strain by Genomic DNA Mutagenesis[J]. Chemistry and Industry of Forest Products, 2015, 35(6): 108-112.

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Breeding of Robust Inhibitor-tolerant Industrial Saccharomyces cerevisiae Strain by Genomic DNA Mutagenesis

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