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Chemistry and Industry of Forest Products ›› 2015, Vol. 35 ›› Issue (6): 126-132.doi: 10.3969/j.issn.0253-2417.2015.06.021

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Extraction of Essential Oil from the Flower of Chionanthus retusus Lindl.et Paxton by Supercritical CO2 and Its Antibacterial Activities

LIU Pu, LI Xiao-fang, GAO Jia-yu, YIN Wei-ping, DENG Rui-xue   

  1. Henan Engineering Technology Research Center for Funiu Mountain Wild Medicinal Source, Chemical Engineering & Pharmaceutical College, Henan University of Science and Technology, Luoyang 471023, China
  • Received:2014-10-09 Online:2015-12-25 Published:2015-12-28

Abstract: Single factor experiment and orthogonal experiment design were used to study the influences of extraction conditions on the yield of essential oil from the flower of Chionanthus retusus Lindl.et Paxton by supercritical CO2 method.The components of the essential oil were analyzed by gas chromatography-mass spectrometry (GC-MS).The in vitro antibacterial activity of the eessential oil was characterized.The optimum extraction parameters were established with the extraction pressure 25 MPa, extraction temperature 45 ℃, extraction time 2.0 h, and the fluent of CO2 22 kg/h.The yield of essential oil was up to 0.409%.Sixty compositions were separated.Among them, 57 components were identified and respresented 96.18% of the total detected constituents.The results of the antibacteria, i.e., assay showed that the essential oil displayed good antibacterial activity against the tested bacterial, i.e. Staphyloccocus aureu, Staphyloccocus albus, Escherichia coli.

Key words: Chionanthus retusus Lindl. et Paxton, essentyial oil, supercritical extraction, antibacterial activity

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