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Chemistry and Industry of Forest Products ›› 2016, Vol. 36 ›› Issue (6): 1-8.doi: 10.3969/j.issn.0253-2417.2016.06.001

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Separation and Identification of Phenolic Compounds of Ethanol Extract from Lacquertree Woods

CHEN Hong-xia1, WANG Cheng-zhang1,2, ZHOU Hao1,2, TAO Ran1, YE Jian-zhong1, LI Wen-jun1   

  1. 1. Institute of Chemical Industry of Forest Products, CAF, National Engineering Lab. for Biomass Chemical Utilization, Key and Open Lab. of Forest Chemical Engineering, SFA, Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
    2. Research Institute of Forestry New Technology, CAF, Beijing 100091, China
  • Received:2016-02-01 Online:2016-12-25 Published:2016-12-23

Abstract: The aim of this study was to separate antityrosinase compounds of the ethyl acetate fraction of ethanol extract from Toxicodendron vernicifluum (Stokes) F. A. Barkley(lacquertree) wood power using silica-gel column chromatography, medium pressure liquid chromatography and preparative high performance liquid chromatography through bioactivity-guided isolation. Seven phenolic compounds were obtained and identified from the ethyl acetate fraction from lacquertree by IR, MS and NMR chromatographies. The separated compounds were gallic acid(1), fustin(2), fisetin(3), sulfuretin(4), 3,4',7-trihydroxy-flavanonol(5), quercetin(6) and butin(7). The antityrosinase activities(IC50) from strong to weak were sulfuretin(3.74 mg/L), butin(4.32 mg/L), quercetin(18.73 mg/L), fisetin(69.4 mg/L), gallic acid(89.5 mg/L), fustin(100 mg/L) and 3,4',7-trihydroxy-flavanonol(164.07 mg/L).

Key words: ethyl acetate fraction, phenolic compounds, middle pressure chromatography, antityrosinase activity

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