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Chemistry and Industry of Forest Products ›› 2016, Vol. 36 ›› Issue (6): 100-106.doi: 10.3969/j.issn.0253-2417.2016.06.016

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Enzyme-assisted Extraction and Macroporous Resin Purification of Fustin from Rhus punjabensis var. Sinica Rehd. Fruit

DONG Ai-wen, YAO Shu-feng, ZHAO Hong-qiao, CHENG Jiang, WU Ling   

  1. Forest Products Chemical Engineering Key Laboratory of Hunan Province, Jishou University, Zhangjiajie 427000, China
  • Received:2016-02-17 Online:2016-12-25 Published:2016-12-23

Abstract: After the Rhus punjabensis var.sinica fruits were disposed by cellulase, the volume fraction of 70% ethanol-macroporous resin combined technique was used to extract and purify fustin. With the fustin yield as study index, the orthogonal experiment was employed to optimize the extraction process. The best resin for fustin separation and purification was screened from eight kinds of macroporous resin to study the effect on the static and dynamic adsorption and desorption of fustin. The results showed that the optimum conditions for the enzyme treatment were enzyme dosage 120 U/mL, enzymolysis temperature 55℃, enzymolysis time 60 min and enzymolysis pH value 4.5, and the fustin yield was up to 1.35% with the purity of 14.59%. Through Static adsorption test, the most effective XDA-8 resin was selected. The dynamic adsorption test showed that the best conditions for XDA-8 separation and purification were sample mass concentration of 14.4 g/L, flow rate of 80.384 mL/h, ethanol volume fraction of 60% and eluent flow rate of 80.384 mL/h. Under these conditions, the desorption rate was 91.86% and after purification the fustin purity could reach 68.15%.

Key words: Rhus punjabensis var.sinica Rehd. fruit, cellulase, dihydrofisetin, macroporous adsorption resin, HPLC

CLC Number: