响应面法优化超声波辅助乙醇/盐双水相萃取栀子中京尼平苷

doi:10.3969/j.issn.0253-2417.2017.02.012

Abstract

Abstract: An ultrasonic-assisted technology combined with ethanol/NaH₂PO₄ aqueous two-phase system (UAATPE) was developed for the extraction and purification of geniposide (GP) from gardenia fruits. Based on the results of single factor experiments, the Box-Behnken Design combined with response surface methodology was applied to optimize the operating parameters. Furthermore, the comparisons with ultrasonic solvent extraction, heat reflux extraction, and ultrasonic-assisted aqueous two-phase extraction were also assessed. GP was mainly extracted into the top ethanol-rich phase. And the optimal experimental conditions were 10.0 g ethanol/salt aqueous two-phase system, NaH₂PO₄ mass fraction 15.00%, ethanol 4 mL(ethanol mass fraction 31.56%), extraction temperature 30 ℃, extraction time 30 min and sample loading 0.03 g. And the maximum yield of GP in top phase was 51.77 mg/g. The comparison results indicated that ultrasonic-assisted aqueous two-phase extraction was more efficient and environmentally friendly than ultrasonic solvent extraction and heat reflux extraction, for the technique had the shorter extraction time, the lower extraction temperature and the higher extraction efficiency.

Key words: aqueous two-phase systems, ultrasonic assisted, gardenia fruits, geniposide

CLC Number:

TQ35

LIU Leilei, ZHAO Shuai, WANG Huan, PENG Sheng, GUO Jie. Optimization of Ultrasonic Assisted Extraction of Geniposide from Gardenia Fruits by Using Ethanol/Salt Aqueous Two-phase System with Response Surface Method[J]. Chemistry and Industry of Forest Products, 2017, 37(2): 93-100.

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Optimization of Ultrasonic Assisted Extraction of Geniposide from Gardenia Fruits by Using Ethanol/Salt Aqueous Two-phase System with Response Surface Method

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