金属离子对花椒毒酚结合牛血清白蛋白体系的影响

doi:10.3969/j.issn.0253-2417.2017.05.010

Abstract

Abstract: The mechanisms of the interaction between xanthotoxol(XAL) and bovine serum albumin(BSA) were investigated by fluorescence and circular dichroism(CD) spectrometry, as well as the effects of common metal ions(Fe³⁺, Cu²⁺, Zn²⁺, Cr³⁺) on the XAL-BSA binding. The quenching constants (k_q) calculated by Stern-Volmer equation under 290,297 and 304 K were 6.316×10¹³,4.402×10¹³ and 3.554×10¹³ L/(mol·s), respectively. The result indicated that XAL could quench the intrinsic fluorescence of BSA strongly, and the quenching mechanism was static quenching process. The binding constant (K) of XAL to BSA at 297 K was 3.47×10⁵ L/mol. The addition of metal ions changed the binding constant. The distance between the tryptophan residues in BSA and XAL was 5.29 nm by using Föster's equation, and it decreased after interaction with metal ions. The CD spectrometry demonstrated that the secondary structure of BSA changed after its interaction with XAL, and α-helix content decreased; the secondary structure of BSA was also changed by addition of metal ions and α-helix content further reduced.

Key words: xanthotoxol, bovine serum albumin, interaction

CLC Number:

TQ35
O657.3

CAO Hongkun, JIN Xiaowei, ZHANG Enfeng, YU Hui, SHI Jingnan, BIAN Hedong. Interactions Between Drugs and Bovine Serum Albumin in the Presence of Metal Ions by Spectral Methods[J]. Chemistry and Industry of Forest Products, 2017, 37(5): 79-87.

References

[1] 张新勇,向仁德. 蛇床子化学成分的研究[J]. 中草药,1997(10):588-590. ZHANG X Y,XIANG R D. Study on the chemical constituents of osthol[J].Chinese Traditional and Herbal Drugs,1997(10):588-590.
[2] 张海蓉,边贺东,潘英明,等. 光谱法研究儿茶素与牛血清白蛋白的相互作用[J]. 光谱学与光谱分析,2009,29(11):3052-3056. ZHANG H R,BIAN H D,PAN Y M,et al.Investigation of interaction between catechin and bovine serum albumin by spectroscopic methods[J]. Spectroscopy and Spectral Analysis,2009,29(11):3052-3056.
[3] ZHANG J,YAN Q,LIU J,et al. Study of the interaction between 5-sulfosalicylic acid and bovine serum albumin by fluorescence spectroscopy[J]. Journal of Luminescence,2013,134:747-753.
[4] 易平贵,商志才,俞庆森,等. 丝裂霉素C与牛血清白蛋白结合作用的研究[J]. 化学学报,2000,58(12):1649-1653. YI P G,SHANG Z C,YU Q S,et al.Studies on the interaction between mytomycin C and bovine serum albumin[J].Acta Chimica Sinica,2000,58(12):1649-1653.
[5] ZHONG W,WANG Y,YU J S,et al. The interaction of human serum albumin with a novel antidiabetic agent-SU-118[J]. Journal of Pharmaceutical Sciences,2004,93:1039-1046.
[6] SHAHABADI N,MOHAMMADPOUR M. Study on the interaction of sodium morin-5-sulfonate with bovine serum albumin by spectroscopic techniques[J]. Spectrochimica Acta Part A Molecular & Biomolecular Spectroscopy,2012,86(3):191-195.
[7] 冷晓莲,杨娟,苏波,等. 芦丁对胃蛋白酶部分性质的影响[J]. 天然产物研究与开发,2007,19(2):270-273. LENG X L,YANG J,SU B,et al. The partial influence of rutin on pepsin[J].Natural Product Research and Development,2007,19(2):270-273.
[8] WARE W R. Oxygen quenching of fluorescence in solution:An experimental study of the diffusion process[J]. Journal of Physical Chemistry,1962,66(3):316-320.
[9] CYRIL L. Biochemists' Handbook[M]. London:E. & F. N. Spon Ltd.,1961.
[10] RAHNAMA E,MAHMOODIAN-MOGHADDAM M,KHORSAND-AHMADI S,et al. Binding site identification of metformin to human serum albumin and glycated human serum albumin by spectroscopic and molecular modeling techniques:A comparison study[J]. Journal of Biomolecular Structure and Dynamics,2015,33(3):513-533.
[11] 邵爽,邱瑾. 金属离子对齐多夫定与牛血清白蛋白结合作用的影响[J]. 物理化学学报,2009,25(7):1342-1346. SHAO S,QIU J,Effecting of Metal ions on the interaction between zidovudine and bovine serum albumin[J].Acta Physico-Chimica Sinica,2009,25(7):1342-1346.
[12] YING L,HE W,LIU J,et al. Binding of the bioactive component Jatrorrhizine to human serum albumin[J]. Biochimica Et Biophysica Acta,2005,1722(1):15-21.
[13] REHMAN M T,SHAMSI H,KHAN A U. Insight into the binding mechanism of imipenem to human serum albumin by spectroscopic and computational approaches[J]. Molecular pharmaceutics,2014,11(6):1785-1797.
[14] YUE Y,ZHANG Y,ZHOU L,et al. In vitro study on the binding of herbicide glyphosate to human serum albumin by optical spectroscopy and molecular modeling[J]. Journal of Photochemistry & Photobiology B Biology,2008,90(1):26-32.
[15] LIU J,TIAN J,HE W,et al. Spectrofluorimetric study of the binding of daphnetin to bovine serum albumin[J]. Journal of Pharmaceutical & Biomedical Analysis,2004,35(3):671-677.
[16] GVLER G,VOROB'EV M M,VOGEL V,et al. Proteolytically-induced changes of secondary structural protein conformation of bovine serum albumin monitored by Fourier transform infrared(FT-IR) and UV-circular dichroism spectroscopy[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2016,161:8-18.
[17] HU Y J,LIU Y,ZHANG L X,et al. Studies of interaction between colchicine and bovine serum albumin by fluorescence quenching method[J]. Journal of Molecular Structure,2005,750(1/2/3):174-178.

Interactions Between Drugs and Bovine Serum Albumin in the Presence of Metal Ions by Spectral Methods

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