Welcome to Chemistry and Industry of Forest Products,

Chemistry and Industry of Forest Products ›› 2017, Vol. 37 ›› Issue (5): 101-106.doi: 10.3969/j.issn.0253-2417.2017.05.013

Previous Articles     Next Articles

Rapid and Nondestructive Identification of Dendrobium huoshanense Fengdou and Dendrobium henanense Fengdou by Portable NIR Spectrometer

JIANG Mingzhu1,2, HAN Bangxing3,4, YAN Hui2, JIA Junqiang2, TU Jie2, MA Yongkun1   

  1. 1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;
    2. School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China;
    3. School of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, China;
    4. Chinese Traditional Medicine Resource Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2017-01-05 Online:2017-10-25 Published:2017-10-30

Abstract: The near infrared spectra of 231 samples of Dendrobium huoshanense and Dendrobium henanense were measured by portable near infrared spectroscopy. Nine kinds of preprocessing methods, such as first derivative(1stD), standard normal variate(SNV), mean centering(MNCN), multiplicative signal correction(MSC),normalize(N) and their combination were used to pretreat original spectrometer. The model of identifying D. huoshanense and D. henanense were established with PLS-DA method. Different spectral preprocessing methods were compared with the accuracy of model. The models were also evaluated with the accuracy, sensitivity and specificity of PLS-DA model. The results showed that the 1stD+SNV+MNCN preprocessing method had the best effect, the accuracy of the model was the highest. When the latent variable was 12, the prediction accuracy of calibration set, cross validation set and prediction set were 100%, and 100% specificity and sensitivity rate of PLS-DA model were also obtained. The results indicted that PLS-DA analysis with Near-Infrared can provide a new method for rapid and non-invasive identification of D. huoshanense Fengdou and D. henanense Fengdou.

Key words: portable NIR spectrometer, PLS-DA, Dendrobium huoshanense Fengdou, Dendrobium henanense Fengdou, nondestructive identification

CLC Number: