隐孔菌中倍半萜类化合物及其抗氧化活性研究

doi:10.3969/j.issn.0253-2417.2018.01.011

Abstract

Abstract: The antioxidant activities of four extracts (alkali extracts,ethyl acetate extract,60% ethanol extract and crude polysaccharide) from Cryptoporus volvatus(Peck) Hubbard were evaluated by methods of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical,2,2'-azino-di-(3-ethyl-2,3-dihydrobenzthiazoline-6-sulfonate)(ABTS),Salicylic acid,and Fenton reaction.The results indicated that ethyl acetate extract showed the strongest free radical scavenging ability with IC₅₀=(67.43±1.41) mg/L by the ABTS method.Further study by bioassay-guided fractionation of the ethyl acetate extract led to the isolation and identification of nine cryptoporic compounds including cryptoporic acid A trimethyl ester (1),cryptoporic acid A (2),cryptoporic acid D tetra-methyl ester (3),cryptoporic acid D (4),cryptoporic acid C (5),cryptoporic acid C pentamethyl ester (6),cryptoporic acid E pentamethyl ester (7),cryptoporic acid B trimethyl ester (8) and crytoporic acid B (9).Among them,compounds 1,3,6,8 were isolated for the first time from this fungus.The results of the ABTS scavenging ability showed that compound 8(cryptoporic acid B trimethyl ester) has a higher antioxidant activity with IC₅₀=(5.29±0.25) mg/L than the positive control V_C with IC₅₀=(5.76±0.58) mg/L.In addition,the structure-relationship of sesquiterpenes as antioxidant agents was analyzed.

Key words: Cryptoporus volvatus, sesquiterpenes, antioxidant activities, bioassay-guided fractionation, structure-activity relationship

CLC Number:

TQ35
TQ91

ZHOU Lingyun, LU Bin, YU Xiaohong, ZHANG Mingrui, HUA Yan. Antioxidant Activity of Sesquiterpenes from Cryptoporus volvatus (Peck) Hubbard[J]. Chemistry and Industry of Forest Products, 2018, 38(1): 73-80.

References

[1] 赵维鼎. 中国真菌志:第三卷[M]. 北京:科学出版社,1998:97. ZHAO J D. Flora Fungorum Sinicorum:Volume 3[M]. Beijing:Science Press,1998:97.
[2] WU S H,ZANG M. Cryptoporus sinensis sp.nov.,a new polypore found in China[J]. Mycotaxon,2000,74(2):415-422.
[3] 应建浙. 中国药用真菌图鉴[M]. 北京:科学出版杜,1987:212. YING J Z. Chinese Medicinal Fungi Illustration[M]. Beijing:Science Press,1987:212.
[4] WERNS S W,SHEA M J,LUCCHESI B R. Free radicals and myocardial injury:Pharmacologic implications[J]. Circulation,1986,74(1):1-5.
[5] ASAKAWA Y,HASHIMOTO T,MIZUNO Y,et al. Cryptoporic acids A-G,drimane-type sesquiterpenoid ethers of isocitric acid from the fungus Cryptoporus volvatus[J]. Phytochemistry,1992,31(2):579-592.
[6] WANG J C,LI G Z,LV N,et al. Cryptoporic acid S,a new drimane-type sesquiterpene ether of isocitric acid from the fruiting bodies of Cryptoporus volvatus[J]. Journal of Asian Natural Products Research,2017,19(7):719-724.
[7] 张华,周志钦,席万鹏. 15种柑橘果实主要酚类物质的体外抗氧化活性比较[J]. 食品科学,2015,36(11):64-70. ZHANG H,ZHOU Z Q,XI W P. Comparison of antioxidant activity in vitro of 15 major phenolic compounds in citrus fruits[J]. Food Science,2015,36(11):64-70.
[8] 乐世俊,唐于平,王林艳,等. 红花中黄酮类化合物的分离与体外抗氧化研究[J]. 中国中药杂志,2014,39(17):3295-3300. LE S J,TANG Y P,WANG L Y,et al. Separation and evaluation of antioxidant constituents from Carthamus tinctorius[J]. Chinese Journal of Chinese Materia Medica,2014,39(17):3295-3300.
[9] 谭晓虹,田嘉铭,赵江垒,等. 蛇床子素体外抗氧化活性研究[J]. 中成药,2013,35(5):1070-1072. TAN X H,TIAN J M,ZHAO J L,et al. Study on in vitro antioxidant activity of Cnidium monnieri[J]. Chinese Traditional Patent Medicine,2013,35(5):1070-1072.
[10] 孙惠芳,马瑞丽,徐秀泉,等. 大血藤抗氧化及抗菌活性研究[J]. 中国野生植物资源,2015,34(3):16-19. SUN H F,MA R L,XU X Q,et al. Antioxidant and antibacterial properties of Sargentodoxa cuneata [J]. Chinese Wild Plant Resources,2015,34(3):16-19.
[11] 翁文江,高雪. 火棘果不同极性部位提取物体外抗氧化研究[J]. 食品工业科技,2015,36(1):77-80. WENG W J,GAO X. Study on antioxidant activity in vitro about different solvent extracts of fruits of Pyracantha fortuneana [J]. Science and Technology of Food Industry,2015,36(1):77-80.
[12] 韦玉兰,苏上贵,黄燕军,等. 金樱子多糖抗氧化作用的实验研究[J]. 广西中医药,2015,38(3):61-64. WEI Y L,SU S G,HUANG Y J,et al. Experiment study on antioxidant capacity of Rosa laevigata Michx polysaccharide in vitro [J]. Guangxi Journal of Traditional Chinese Medicine,2015,38(3):61-64.
[13] 韩少华,朱靖博,王妍妍. 邻苯三酚自氧化法测定抗氧化活性的方法研究[J]. 中国酿造,2009,41(6):155-157. HAN S H,ZHU J B,WANG Y Y. Measurement of the antioxidant activity by pyrogallol autoxidation[J]. China Brewing,2009,41(6):155-157.
[14] 金越,吕勇,韩国柱,等. 槲皮素及异槲皮素、芦丁抗自由基活性的比较研究[J]. 中草药,2007,38(3):408-412. JIN Y,LÜ Y,HAN G Z,et al. Comparative study on in vitro anti-free radical activities of quercetin,isoquercetin,and rutin[J]. Chinese Traditional and Herbal Drugs,2007,38(3):408-412.
[15] WANG J C,LI G Z,GAO L,et al. Two new cryptoporic acid derivatives from the fruiting bodies of Cryptoporus volvatus[J]. Phytochemistry Letters,2015,14:63-66.

Antioxidant Activity of Sesquiterpenes from Cryptoporus volvatus (Peck) Hubbard

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Recommended Articles

Metrics

Comments

[1]	WANG Xingda, LI Lei, HAN Yongping. Synthesis and Antibacterial Activity of Derivatives of Rhein [J]. Chemistry and Industry of Forest Products, 2018, 38(2): 105-111.
[2]	WEI Qiang, JI Xiao-ying, LONG Xian-shun, LI Qian-rong, YIN Hao. Chemical Constituents Extracted from Leaves of Prunus cerasifera Ehrh.cv. Atropurpurea Jacg. and Their Antioxidant Activities in vitro [J]. Chemistry and Industry of Forest Products, 2015, 35(5): 116-122.
[3]	HU Guo-yuan;LI Wei-wei;YUAN Jun;WEI Ling-yun. Modification Technology and Structure-activity Relationship of Poria cocos Polysaccharide [J]. , 2013, 33(5): 121-126.
[4]	WANG Zong-de;SONG Jie;JIANG Zhi-kuan;CHEN Jin-zhu;HAN Zhao-jiu;ZHENG Wei-qing;SONG Zhan-qian;SHANG Shi-bin. Studies on Repellency and Quantitative Structure-activity Relationship of Ant Repellent Derived from Turpentine Oil [J]. , 2009, 29(S1): 47-53.
[5]	NIE Ling-hong;NING Zheng-xiang. RELATIONSHIP BETWEEN STRUCTURE AND ACTIVITY OF ACTIVE POLYSACCHARIDES [J]. , 2003, 23(04): 89-94.