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Chemistry and Industry of Forest Products ›› 2018, Vol. 38 ›› Issue (2): 75-79.doi: 10.3969/j.issn.0253-2417.2018.02.010

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Effect of Pretreatment Methods on Cellulase Adsorption Profiles of Sugarcane Bagasse

DONG Yifan, LIU Zhijia, LAN Tianqing   

  1. Food Safety Institute, Kunming University of Science and Technology, Kunming 650500, China
  • Received:2017-09-01 Online:2018-04-25 Published:2018-04-28

Abstract: The effects of two pretreatment methods, sulfite (SPORL) pretreatment and dilute sulfuric acid (DA) pretreatment, on the enzymatic hydrolysis of sugarcane bagasse (SCB) substrates were investigated and the adsorption of cellulose on SCB during the hydrolysis was studied. The enzymatic hydrolysis efficiency, composition, surface morphology, adsorption isotherms and kinetics of two kinds of pretreated SCB were compared. The results showed that the enzymatic hydrolysis efficiency of SPORL-SCB(81.92%) was significantly higher than that of DA treated SCB(60.89%). Compared with the untreated SCB, the surface of SCB treated by SPORL and DA became rough, the surface fibres were partially stripped and the internal fibre skeleton was exposed. The holocellulose contents of these two treated SCB substrates were not significantly different, but the Klason lignin content of SPORL treated SCB was lower. The XPS images suggested that the treated SCB substrates both adsorbed cellulase. The adsorption isotherm and kinetics curves showed that the maximum adsorption of cellulase on SPORL treated SCB was 61.91 mg/g and lower than that on DA treated SCB(82.69 mg/g), but the adsorption affinity and adsorption strength of SPORL treated SCB were higher than those of the DA treated SCB.

Key words: sugarcane bagasse, sulfite pretreatment, dilute acid pretreatment, enzymatic hydrolysis, cellulase adsorption

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