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Chemistry and Industry of Forest Products ›› 2018, Vol. 38 ›› Issue (2): 80-86.doi: 10.3969/j.issn.0253-2417.2018.02.011

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Isolation and Identification of Antifungal Ingredients from Artemisia hedinii

XU Jie1, CHENG Chuanke1, HU Guoyuan1, YANG Chunlei2, YU Jun2, YANG Jinpeng2   

  1. 1. School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430205, China;
    2. Tobacco Research Institute of Hubei Province, Wuhan 430030, China
  • Received:2017-09-20 Online:2018-04-25 Published:2018-04-28

Abstract: The extraction of Artemisia hedinii Ostenf. et Pauls(A. hedinii) were rough and further isolated by chromatographic separation technology, and the antifungal activity of the component was evaluated by the the spore germination method. Furthermore, the composition of antimicrobial components was analyzed by high performance liquid chromatography-mass spectrometry (HPLC-MS). The results showed that 13 kinds of components(Fr.1-Fr.13) were rough isolated from the extraction of A. hedinii, in which the component Fr.5 showed the best antifungal activity. Four kinds of components (Fr.5.1-Fr.5.4) were obtained by the further separation of the component Fr.5. The EC50 of A. hedinii crude extraction on Alternaria alternate (A.alternate) spores was 5.04 g/L, and that of the component Fr.5.2 was 0.57 g/L. The HPLC-MS results showed that the components of Fr.5.2 mainly contained deoxy double hydroxyl artemether (17%),deoxy dihydroartemisinin (43.65%) and deoxyartemisinin (16.15%). The content of dihydroartemisinin of component Fr.5.2 was calculated as 40.95% by using dihydroartemisinin as the standard. The EC50 of dihydroartemisinin on A.alternate was 2.70 g/L, indicating that the major antifungal matter in component Fr.5.2 was artemisinin derivatives.

Key words: Artemisia hedinii, Alternaria alternate, toxicity, artemisinin derivatives

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