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Chemistry and Industry of Forest Products ›› 2018, Vol. 38 ›› Issue (4): 61-68.doi: 10.3969/j.issn.0253-2417.2018.04.010

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Ultrasonic-assisted Radical Process for Directed Degradation of Arabinogalactan

ZHANG Haiyun1,2, HE Liang2, LI Qin2, YANG Bo1, YANG Guang1   

  1. 1. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;
    2. Zhengjiang Provincial Key Laboratory of Biological and Chemical Utilization of Forest Resources, Zhengjiang Forestry Academy, Hangzhou 310023, China
  • Received:2018-03-19 Online:2018-08-25 Published:2018-08-10

Abstract: Arabinogalactan (AG) was extracted from the sawdust of larch (Larix gmelinii(Rupr.) Kuzen). The arabinogalactan obtained was physically degraded by ultrasound-assisted free radicals. The free radical scavenging ability of decomposed AG was selected as an indicator on the evaluation of the antioxidant activity. On the basis of single-factor test, response surface optimization was used to determine the optimal degradation conditions, and the optimum conditions are found as follows:the dpolysaccharide concentration was 10 g/L; ultrasonic temperature was 60℃; the H2O2 volume fraction was 6%;and the ultrasonic time was 60 min. Under this optimum degradation condition, the·OH scavenging rate of arabinogalactan was 89.6%, and Mr of AG was degraded from 52 ku to around 25 ku, and the cumulative weight fraction of this molecular weight reached 60%.Compared with AG, the antioxidant activity of arabinogalactan oligosaccharide(AG-O) obtained in the anti-oxidative activity was significantly enhanced, and its IC50 value decreased from 2.058 g/L to 0.497g/L.

Key words: arabinogalactan, free radical scavenging, response surface optimizatio

CLC Number: