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Chemistry and Industry of Forest Products ›› 2018, Vol. 38 ›› Issue (5): 100-106.doi: 10.3969/j.issn.0253-2417.2018.05.014

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Pretreatment of Corn Cob by the Combination of H3PO4-H2O2 and Their Effect on Enzymatic Hydrolysis Efficiency

YING Wenjun1, WU Kai1, SHI Zhengjun1,2, YANG Haiyan1,2, ZHENG Zhifeng1,2, YANG Jing1,2   

  1. 1. College of Chemical Engineering, Southwest Forestry University, Kunming 650224, China;
    2. University Key Laboratory of Biomass Chemical Refinery & Synthesis;Engineering Laboratory of Biomass High Efficiency Utilization, Southwest Forestry University, Kunming 650224, China
  • Received:2018-05-16 Online:2018-10-25 Published:2018-11-06

Abstract: Corn cob was pretreated by H3PO4-H2O2(PHP)solution and then hydrolyzed by cellulase. The conditions of pretreatment was optimized, which was based on saccharification rate of substrate. Results showed that in pretreated corn cob under the optimized conditions of pretreatment (mass fraction of H3PO4 80%, 4 h, 50℃), the mass fraction of cellulose was 57.38%; the cellulose recovery, hemicellulose removal and lignin removal were 95.84%, 62.36% and 68.97%, respectively.As the dosage of cellulase was 10 FPIU/g glucan, the yield of 72 h enzymatic hydrolysis was 39.12%, which was 2.61 folds higher than that of the enzymatic hydrolysis yield of corn cob without pretreatment(10.84%). Using FT-IR to analyze the change of structure before and after pretreatment, the results showed that, PHP pretreatment had coordinated pretreatment effect in the pretreatment process, which could remove hemicellulose and lignin at the same time. For comparative purpose, H3PO4 (16.78%) or H2O2 pretreatment (20.71%) was used independently, to enzymatic hydrolysis of corn cob for 72 h. It was found that the yield of 72 h enzymatic hydrolysis treated by H3PO4-H2O2 pretreatment increased by 133.13% and 88.89%, respectively, while compared with that of H3PO4 or H2O2.

Key words: optimization, corn cob, structural changes, cellulose, enzymatic hydrolysis

CLC Number: