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Chemistry and Industry of Forest Products ›› 2019, Vol. 39 ›› Issue (1): 41-45.doi: 10.3969/j.issn.0253-2417.2019.01.006

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Separation, Purification and Hydrophilic Derivative Synthesis of Ginkgo biloba Leaves Polyprenol

Changwei ZHANG1,Yulu MA1,Ran TAO1,Jianzhong YE1,Chengzhang WANG1,2,*()   

  1. 1. Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab. for Biomass Chemical Utilization; Key and Open Lab. of Forest Chemical Engineering, SFA; Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China
    2. Research Institute of Forestry New Technology, CAF, Beijing 100091, China
  • Received:2018-09-05 Online:2019-02-25 Published:2019-03-14
  • Contact: Chengzhang WANG E-mail:wangczlhs@sina.com
  • Supported by:


The analytical methodology and method performance for the determination of polyprenol in Ginkgo biloba leaves were investigated for the first time. The results showed that of the high performance liquid chromatography with C18 as chromatographic column, isopropanol methanol(volume ratio, 32:18) as mobile phase, 210 nm as detection wavelength showed high precision(RSD=0.89%) and good stability(RSD=3.26%). In addition, the average standard recovery and RSD value of polyprenol were 97.4% and 1.75%, respectively. Then, polyprenol with the purity of 98.6% was obtained by extraction with n-hexane, saponification, de-waxing with ethanol and acetone, and silica gel column chromatography(V(diethyl ether):V(n-hexane)=3:97). On this basis, the terminal hydroxyl group of high-purity polyprenol was modified to synthesize aminopolyprenol. Firstly, by mitsunobu reaction with phthalimide, followed by reduction reaction with hydrazine hydrate, to obtain aminopolyprenol. The structure of amino polyprenol was characterized by infrared and nuclear magnetic resonance spectroscopy, which confirmed the aminopolyprenol was successfully synthesized.

Key words: polyprenol, separation, purification, hydrophilic modification

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