Chemistry and Industry of Forest Products ›› 2019, Vol. 39 ›› Issue (1): 88-94.doi: 10.3969/j.issn.0253-2417.2019.01.013
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Jinjin WEI,Donglin XIN,Xiang CHEN,Xiyu QUAN,Junhua ZHANG*()
Received:
2018-10-16
Online:
2019-02-25
Published:
2019-03-14
Contact:
Junhua ZHANG
E-mail:junhuazhang@nwafu.edu.cn
Supported by:
CLC Number:
Jinjin WEI,Donglin XIN,Xiang CHEN,Xiyu QUAN,Junhua ZHANG. Effect of Steam Explosion on Extraction of Bioactive Components and Gutta-percha from Barks of Eucommia ulmoides Oliver[J]. Chemistry and Industry of Forest Products, 2019, 39(1): 88-94.
Table 1
Effect of extraction solvents on yields of active compounds from untreated barks of E. ulmoides Oliver"
提取溶剂extraction solvent | 黄酮/(mg·g-1)flavonoids | 桃叶珊瑚苷/(mg·g-1)aucubin | 京尼平苷酸/(mg·g-1)geniposidic acid | 绿原酸/(mg·g-1)chlorogenic acid |
缓冲液buffer | 5.93±0.00 | 0.98±0.15 | 0.54±0.01 | 0.24±0.05 |
纤维素酶cellulase | 7.52±0.16 | 1.13±0.10 | 0.35±0.01 | 0.11±0.03 |
水water | 5.08±0.07 | 0.70±0.34 | 0.45±0.08 | 0.10±0.04 |
30%乙醇30% ethanol | 6.53±0.14 | 1.81±0.00 | 0.63±0.04 | 0.34±0.02 |
60%乙醇60% ethanol | 6.07±0.05 | 1.66±0.05 | 0.72±0.01 | 0.24±0.08 |
Table 2
Effect of extraction conditions on yields of active compounds from untreated barks of E. ulmoides Oliver"
提取条件 extraction condition | 黄酮/(mg·g-1) flavonoids | 桃叶珊瑚苷/(mg·g-1 aucubin | 京尼平苷酸/(mg·g-1) geniposidic acid | 绿原酸/(mg·g-1) chlorogenic acid | |
乙醇体积分数volume fraction of ethanol | 20% | 5.74±0.23 | 7.74±0.44 | 0.54±0.01 | 0.20±0.01 |
30% | 6.29±0.02 | 9.26±0.65 | 0.69±0.05 | 0.21±0.00 | |
40% | 7.01±0.11 | 11.55±0.65 | 0.62±0.03 | 0.24±0.01 | |
50% | 6.50±0.23 | 8.23±0.71 | 0.75±0.08 | 0.26±0.04 | |
60% | 6.31±0.50 | 8.45±0.16 | 0.71±0.01 | 0.27±0.05 | |
70% | 7.15±0.21 | 25.53±0.92 | 0.78±0.02 | 0.26±0.00 | |
提取时间extraction time | 20 min | 5.59 ±0.02 | 7.36±0.60 | 0.67±0.06 | 0.23±0.01 |
40 min | 5.70±0.13 | 7.74±0.22 | 0.59±0.02 | 0.28±0.03 | |
60 min | 3.47±0.07 | 8.45±0.60 | 0.58±0.01 | 0.29±0.00 | |
80 min | 4.27±0.29 | 9.64±0.71 | 0.59±0.00 | 0.25±0.01 | |
100 min | 3.89±0.35 | 8.72±0.87 | 0.58±0.10 | 0.28±0.02 | |
120 min | 3.65±0.11 | 8.12±0.27 | 0.54±0.03 | 0.22±0.00 | |
超声波功率ultrasonic power | 100 W | 6.33±0.48 | 9.15±0.54 | 0.64±0.04 | 0.25±0.01 |
200 W | 6.77±0.13 | 9.53±0.27 | 0.59±0.02 | 0.28±0.04 | |
300 W | 5.85±0.13 | 9.86±0.27 | 0.58±0.00 | 0.19±0.00 | |
400 W | 6.37±0.09 | 10.02±0.22 | 0.64±0.01 | 0.24±0.00 | |
500 W | 6.02±0.13 | 9.53±0.49 | 0.59±0.03 | 0.21±0.01 | |
提取温度extraction temperature | 25 ℃ | 7.66±0.05 | 6.22±0.65 | 0.61±0.02 | 0.29±0.06 |
40 ℃ | 7.63±0.08 | 7.30±1.09 | 0.69±0.06 | 0.25±0.01 | |
55 ℃ | 9.11±0.45 | 7.79±0.82 | 0.64±0.03 | 0.29±0.00 | |
65 ℃ | 9.34±0.28 | 7.85±0.65 | 0.65±0.01 | 0.29±0.04 |
Table 3
Effect of steam explosion on yields of active compounds from barks of E. ulmoides Oliver"
预处理pretreatment | 黄酮/(mg·g-1)flavonoids | 桃叶珊瑚苷/(mg·g-1)aucubin | 京尼平苷酸/(mg·g-1)geniposidic acid | 绿原酸/(mg·g-1)chlorogenic acid | |
压力/MPapressure | 时间/mintime | ||||
0 | 0 | 7.63±0.08 | 7.30±1.09 | 0.69±0.06 | 0.25±0.01 |
0.52 | 8 | 5.08±0.46 | 1.23±0.41 | 0.44±0.11 | 0.23±0.01 |
0.52 | 15 | 3.85±0.33 | 0.93±0.49 | 0.33±0.02 | 0.32±0.01 |
0.90 | 6 | 8.30±1.75 | 1.15±0.16 | 0.24±0.04 | 0.21±0.05 |
1.45 | 3 | 14.04±1.23 | 1.83±0.08 | 0.36±0.12 | 0.41±0.03 |
1.45 | 8 | 17.68±0.83 | 2.18±0.49 | 0.49±0.06 | 0.73±0.01 |
2.10 | 6 | 21.82±0.42 | 2.16±0.14 | 0.29±0.08 | 1.01±0.06 |
3.25 | 1 | 26.83±0.04 | 2.46±0.05 | 0.33±0.03 | 1.22±0.02 |
Table 4
Contents of active compounds and saccharides from barks of E. ulmoides Oliver in the supernatant of enzymatic hydrolysis"
预处理 pretreatment | 活性成分/(mg·g-1) active compounds | 糖得率/(mg·g-1) yield of saccharide | |||||||
压力/ MPapressure | 时间/ mintime | 黄酮 flavonoids | 桃叶珊瑚苷 aucubin | 京尼平苷酸 geniposidic acid | 绿原酸 chlorogenic acid | 葡萄糖glucose | 木糖xylose | ||
0 | 0 | 10.12±0.04 | 1.78±0.03 | 0.00±0.00 | 0.37±0.01 | 97.86±0.72 | 22.75±0.08 | ||
0.52 | 8 | 10.41±0.07 | 0.99±0.04 | 0.13±0.01 | 0.67±0.00 | 111.11±4.11 | 21.81±0.24 | ||
0.52 | 15 | 8.44±0.13 | 0.80±0.06 | 0.11±0.02 | 0.60±0.02 | 103.46±3.02 | 27.00±0.14 | ||
0.90 | 6 | 9.98±0.04 | 0.55±0.01 | 0.02±0.01 | 0.50±0.00 | 110.56±4.15 | 31.21±0.92 | ||
1.45 | 3 | 12.36±0.20 | 0.68±0.03 | 0.03±0.00 | 0.56±0.02 | 124.89±19.46 | 35.63±3.88 | ||
1.45 | 8 | 13.86±0.10 | 0.63±0.05 | 0.00±0.00 | 0.24±0.24 | 102.76±2.47 | 30.34±0.06 | ||
2.10 | 6 | 16.86±0.22 | 0.50±0.03 | 0.07±0.00 | 0.88±0.00 | 104.57±2.16 | 35.61±0.07 | ||
3.25 | 1 | 19.17±0.25 | 0.53±0.02 | 0.09±0.01 | 0.93±0.02 | 100.45±2.93 | 36.41±1.52 |
Table 5
Effect of pretreatment conditions on molecular weight and yield of gutta-percha"
压力/MPapressure | 时间/mintime | Mw | Mn | D=Mw/Mn | 杜仲胶得率/%yield of gutta-percha |
0 | 0 | 42.70×104 | 16.36×104 | 2.61 | 4.82 |
0.52 | 8 | 29.36×104 | 7.58×104 | 3.87 | 5.36 |
0.52 | 15 | 28.39×104 | 6.45×104 | 4.40 | 5.92 |
0.90 | 6 | 26.64×104 | 6.76×104 | 3.94 | 4.73 |
1.45 | 3 | 24.48×104 | 5.74×104 | 4.26 | 4.36 |
1.45 | 8 | 29.64×104 | 7.21×104 | 4.11 | 4.97 |
2.10 | 6 | 33.58×104 | 9.92×104 | 3.39 | 4.35 |
3.25 | 1 | 32.35×104 | 8.47×104 | 3.82 | 4.61 |
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