金属离子对豆腐果苷与牛血清白蛋白相互作用的影响

doi:10.3969/j.issn.0253-2417.2019.01.017

Abstract

Abstract:

The effect of Fe³⁺, Cu², Ni²⁺, Zn²⁺on the interaction reaction between helicid(HLD)and bovine serum albumin(BSA)was investigated by fluorescence spectroscopy under similarity physiological conditions. The results showed that the fluorescence of BSA was quenched via HLD by forming stable complexes. The quenching constants(k_q)calculated by Stern-Volmer equation under 293, 298 and 303 K were 6.316, 5.147 and 4.040×10¹² L/(mol·s), respectively. The binding constants(K) were 9.10×10⁵, 7.76×10⁵, and 4.97 ×10⁵ L/mol, respectively. The distance between the tryptophan residues of BSA and HLD was 4.79 nm by using Föster′s equation, and it decreased after interaction with metal ions. The theromodynamic parameters indicated that the force between HLD and BSA were hydrogen band and van der Waals forces. The effects of HLD and different metal ions on the secondary structure of BSA were studied using circular dichroism(CD) spectroscopy. The results showed that the addition of HLD changed the conformation of BSA in the binding reaction, and α-helix content decreased. The secondary structure of BSA was also changed by the addition of metal ions and α-helix content further reduced obviously.

Key words: bovine serum albumin, helicid, metal ions, interaction

CLC Number:

TQ35

Dandan ZHAO,Xiaowei JIN,Jingnan SHI,Yuzhen ZHANG,Hedong BIAN,Yongzhi LIAO. Effect of Metal Ions on Interaction Between Helicid and Bovine Serum Albumin[J]. Chemistry and Industry of Forest Products, 2019, 39(1): 115-122.

Figures/Tables 13

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References 16

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T/K	体系systems	10^-4K_SV/(L·mol^-1)	10^-12kq/(L·mol^-1·s^-1)	R
293	HLD-BSA	6.316	6.316	0.9867
	HLD-BSA	5.147	5.147	0.9866
	HLD-BSA-Fe³⁺	7.874	7.874	0.9935
298	HLD-BSA-Cu²⁺	6.116	6.116	0.9957
	HLD-BSA-Zn²⁺	5.131	5.131	0.9975
	HLD-BSA-Ni²⁺	9.385	9.385	0.9832
303	HLD-BSA	4.040	4.040	0.9834

T/K	体系systems	10^-5K/(L·mol^-1)	f_a	R
293	HLD-BSA	9.10	0.36	0.9955
	HLD-BSA	7.76	0.35	0.9908
	HLD-BSA-Fe³⁺	2.68	0.56	0.9912
298	HLD-BSA-Cu²⁺	2.16	0.54	0.9970
	HLD-BSA-Zn²⁺	2.32	0.56	0.9990
	HLD-BSA-Ni²⁺	2.84	0.61	0.9922
303	HLD-BSA	4.97	0.34	0.9842

体系system	J/(×10^-14 cm³·L·mol^-1)	R₀/nm	E	r/nm
BSA-HLD-Fe³⁺	6.34	3.35	0.186	4.48
BSA-HLD-Cu²⁺	6.15	3.35	0.097	4.37
BSA-HLD-Zn²⁺	6.83	3.35	0.088	4.58
BSA-HLD-Ni²⁺	6.64	3.35	0.079	4.66

T/K	K/(×10⁵ L·mol^-1)	ΔG^{${\rm{\rlap{-} o}}$}/(kJ·mol^-1)	ΔH^{${\rm{\rlap{-} o}}$}/(kJ·mol^-1)	ΔS^{${\rm{\rlap{-} o}}$}/(J·mol^-1K^-1)
293	9.14	-134.71
298	7.49	-119.42	-224.281	-673.17
303	4.97	-101.02

C_M:C_HLD:C_BSA	Fe³⁺-BSA-HLD/%	Cu²⁺-BSA-HLD/%	Zn²⁺-BSA-HLD/%	Ni²⁺-BSA-HLD/%
0:0:1	40.25	40.25	40.25	40.25
0:1:1	40.03	40.03	40.03	40.03
1:1:1	39.30	39.70	38.39	38.60
2:1:1	37.93	37.66	37.64	38.01

Effect of Metal Ions on Interaction Between Helicid and Bovine Serum Albumin

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