Welcome to Chemistry and Industry of Forest Products,

Chemistry and Industry of Forest Products ›› 2019, Vol. 39 ›› Issue (2): 59-66.doi: 10.3969/j.issn.0253-2417.2019.02.009

Previous Articles     Next Articles

Extraction, Purification and Property Analysis of Polysaccharides from Quercus acutissima

Liming ZHANG1,Rongxue LI1,Xihong HE1,Limin HAO1,2,*(),Zhina HE1,Yujie DAI1   

  1. 1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education; College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
    2. Quartermaster Engineering Technology Institute, Academy of Military Sciences PLA China, Beijing 100027, China
  • Received:2018-11-14 Online:2019-04-25 Published:2019-05-08
  • Contact: Limin HAO E-mail:hlm2005@163.com
  • Supported by:


In order to develop and utilize the polysaccharide from Quercus acutissima, the process parameters of hot water extraction of polysaccharides were studied and the purified polysaccharide was preliminarily analyzed. The extraction conditions including extraction time, temperature, the liquid to solid ratio and times of extraction were investigated by single factor experiment and response surface method with the yield of polysaccharides as index. The optimal extraction conditions were the extraction time 3h, the extraction temperature 90 ℃, the liquid to solid ratio 25:1 (mL:g), and the times of extraction 2. Under these conditions, polysaccharide yield was 2.65%. By using DEAE-52 and Sephadex G-200 column chromatography, two purified polysaccharides (OP-1, OP-2) were obtained. The results of ultraviolet and FT-IR spactra, showed that the purified OP-1 and OP-2 from Q. acutissima had high purity and the typical groups of polysaccharide. The composition analysis by HPLC with pre-column derivatization indicated that the OP-1, a neutral heteropolysaccharide, was mainly composed of xylose and glucose with the molar ratio of 0.86:0.7; the OP-2, an acidic heteropolysaccharide, was mainly composed of ribose, glucuronic acid, and arabinose with the molar ratio of 0.42:0.21:0.56. The results of high performance gel permeation chromatography (HPGPC-ELSD) indicated that the weight average molecular weights (Mw) of OP-1 and OP-2 were about 444.8 and 121.2 ku.

Key words: Quercus acutissima, polysaccharides, extraction technology, response surface analysis, structure characteristics

CLC Number: