水蒸气蒸馏提取芳樟精油及其抑菌活性研究

doi:10.3969/j.issn.0253-2417.2019.03.015

Abstract

Abstract:

In order to investigate the optimal extraction process of Cinnamomum camphora essential oil by steam distillation under laboratory conditions, several factors are investigated by single factor experiments. The results show that the extraction yield of essential oil of the C. camphora leaves is higher than that of branches. The effect of over-water distillation method is better than that of in-water distillation. The optimal distillation power is 600 W, the optimal distillation time is 40 min, and the appropriate post-harvest storage time is less than 48 h. The extraction yield of essential oil under these conditions is 1.42%. The chemical composition of C. camphora essential oil is analyzed by gas chromatograph-mass spectrometer. Six compounds are identified, which account for 94.56% of the total peak area, the main component is linalool, it accounts for 89.58% of the total peak area. The antifungal activities of C. camphora essential oil against six plant pathogenic fungi are tested through determining the growth rate of fungal hyphae. The results show that C. camphora essential oil exhibits good antifungal activity against Rhizoctonia solani, when the testing mass concentration of C. camphora essential oil are 500, 250 and 125 mg/L, the inhibition rates are 100%, 90.88% and 67.94%, respectively.

Key words: Cinnamomum camphora, essential oil, steam distillation, chemical composition, antifungal activity

CLC Number:

TQ35

Hongfu ZHENG,Shengliang LIAO,Guorong FAN,Zongde WANG,Shangxing CHEN,Hongyan SI. Extraction of Cinnamomum camphora Essential Oil by Steam Distillation and Its Antifungal Activity[J]. Chemistry and Industry of Forest Products, 2019, 39(3): 108-114.

Figures/Tables 5

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References 33

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序号 No.	保留时间/min retention time	化合物 compounds	分子式 molecular formula	相对分子质量 molecular weight	GC含量/% GC content	匹配度/% matching degree
1	7.876	芳樟醇linalool	C₁₀H₁₈O	154.1	89.58	98.70
2	15.251	石竹烯caryophyllene	C₁₅H₂₄	204.2	1.06	90.89
3	15.885	蛇麻烯humulene	C₁₅H₂₄	204.2	0.56	81.29
4	16.646	大根香叶烯B germacrene B	C₁₅H₂₄	204.2	0.66	80.39
5	18.054	桉油烯醇spathulenol	C₁₅H₂₄O	220.2	1.96	91.19
6	18.150	石竹烯氧化物caryophyllene oxide	C₁₅H₂₄O	220.2	0.74	80.28

化合物 compounds	质量浓度/ (mg·L^-1) mass concn.	对不同病原菌的抑制率inhibition rate of different pathogens/%
化合物 compounds	质量浓度/ (mg·L^-1) mass concn.	水稻纹枯病菌 R. solani	轮枝镰刀菌 F. verticillioides	彩绒革盖菌 C. versicolor	油茶炭疽病菌 C. gloeosporioides	松枯梢病菌 S. sapinea	七叶树壳梭孢菌 F. aesculi
芳樟精油 C. camphora essential oil	500	100	60	1.23	20.24	10.50	0
	250	90.88	26.49	0	15.48	0	0
	125	67.94	2.78	0	7.74	0	0
	62.50	48.27	0	0	0	0	0
	31.25	39.15	0	0	0	0	0
多菌灵 carbendazim	500	100	100	62.35	100	60.22	100
	250	100	100	65.12	100	54.14	100
	125	100	100	61.42	100	54.70	100
	62.50	100	100	62.65	100	55.25	100
	31.25	100	100	62.96	100	60.22	100

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Extraction of Cinnamomum camphora Essential Oil by Steam Distillation and Its Antifungal Activity

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