肉桂和同源根皮不同极性部位的成分分析及其抑菌效果

doi:10.3969/j.issn.0253-2417.2019.03.016

Abstract

Abstract:

The petroleum ether part, ethyl acetate part, n-butanol part and water part of cinnamon and root bark were obtained by reflux extraction with 80% ethanol and systematic solvent method, using cinnamon and its homologous root bark as raw materials. Their antibacterial activities against three common pathogens(Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa) were evaluated by the filter paper method. The results indicated that the qualities, the types and contents of components of each polar parts extracted from cinnamon and root bark of the same quality were different. The contents of the main components of the two petroleum ether parts were similar, but the contents of trans-cinnamaldehyde and o-methoxycinnamaldehyde, were quite different. Flavonoids, saponins and polysaccharide were found respectively in the two ethyl acetate parts, n-butanol parts and water parts. Moreover, o-methoxy cinnamaldehyde and cinnamyl acetate were found in ethyl acetate part, D-galactose was found in water part of root bark. The petroleum ether part of cinnamon, petroleum ether part and ethyl acetate part of root bark had a certain degree of antibacterial activity against E. coli, S. aureus and P. aeruginosa. When the mass concentrations of these three parts were 500 g/L, the diameters of bacteriostasis circles for S. aureus, E. coli and P. aeruginosa were larger than(17.62±0.22), (25.21±2.09) and(12.82±0.30) mm, respectively. Ethyl acetate part of cinnamon had weaker antibacterial activity, n-butanol part and water part of both cinnamon and root bark had no antibacterial activity.

Key words: cinnamon, root bark, flavonoids, polysaccharide, antibacterial activity

CLC Number:

TQ35
R284

Zuohui ZHANG,Xinyi QIAN,Guangzhi HUANG,Liangwu BI. Component and Antibacterial Activity of Different Polar Parts of Cinnamon and Homologous Root Bark[J]. Chemistry and Industry of Forest Products, 2019, 39(3): 115-122.

Figures/Tables 7

Table 1

Fig.1

Table 2

Fig.2

Fig.3

Fig.4

Table 3

References 30

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g
样品 samples	石油醚部位 petroleum ether part	乙酸乙酯部位 ethyl acetate part	正丁醇部位 n-butanol part	水部位 water part
肉桂cinnamon	11.42	9.32	5.70	35.66
根皮root bark	7.12	26.83	8.42	30.00

序号 No.	t_R/min	化合物 compound	M_r	分子式 fomula	GC含量GC contents/%
序号 No.	t_R/min	化合物 compound	M_r	分子式 fomula	肉桂cinnamon	根皮root bark
1	4.093	苯甲醛benzaldehyde	106	C₇H₆O	—	0.01
2	7.567	苯丙醛benzenepropanal	134	C₉H₁₀O	0.05	0.09
3	8.308	1-氯-2-甲基-2-丙醇1-chloro-2-methyl-2-propanol	108	C₄H₉ClO	—	0.02
4	8.814	顺式肉桂醛cis-cinnamaldehyde	132	C₉H₈O	0.11	0.14
5	10.140	反式肉桂醛trans-cinnamaldehyde	132	C₉H₈O	80.40	46.43
6	10.901	肉桂醇cinnamyl alcohol	134	C₉H₁₀O	—	0.37
7	12.265	古巴烯copaene	204	C₁₅H₂₄	1.55	0.24
8	13.783	香豆素coumarin	146	C₉H₆O₂	1.04	5.89
9	14.015	6-戊基-2H-吡喃-2-酮6-amyl-alpha-pyrone	166	C₁₀H₁₄O₂	0.19	—
10	14.932	α-依兰油烯α-muurolene	204	C₁₅H₂₄	0.73	0.28
11	15.342	δ-杜松烯δ-cadinene	204	C₁₅H₂₄	1.10	0.36
12	15.449	去氢白菖烯calamenene	202	C₁₅H₂₂	0.47	0.09
13	15.636	邻甲氧基肉桂醛o-methoxy cinnamaldehyde	162	C₁₀H₁₀O₂	7.69	40.74
14	17.184	肉豆蔻醛tetradecanal	212	C₁₄H₂₈O	0.21	—
15	20.766	顺式-3-丙酮酸叶醇酯cis-3-hexenylpyruvate	170	C₉H₁₄O₃	0.08	—

样品 samples	部位 different part	质量浓度/(g·L^-1) mass concentration	抑菌圈直径antibacterial cirle diameter /mm
样品 samples	部位 different part	质量浓度/(g·L^-1) mass concentration	金黄色葡萄球菌S.aureus	大肠杆菌E.coli	绿脓杆菌P. aeruginosa
肉桂 cinnamon	石油醚 petroleum ether	500.00	26.49±0.68	27.46±1.19	19.10±1.18
		250.00	17.33±0.57	18.50±1.41	13.76±1.12
		125.00	15.00±0.76	14.19±0.46	10.69±0.34
		62.50	9.71±0.20	12.18±0.73	9.37±0.40
		31.25	9.21±0.10	9.12±0.23	8.96±0.11
	乙酸乙酯 ethyl acetate	500.00	7.76±0.19	14.95±1.30	8.98±0.14
		250.00	—	11.04±0.08	—
		125.00	—	—	—
		62.50	—	—	—
		31.25	—	—	—
根皮 root bark	石油醚 petroleum ether	500.00	19.40±0.22	25.87±1.38	19.70±0.60
		250.00	18.34±0.61	23.97±0.69	17.48±0.74
		125.00	16.16±0.49	19.15±0.38	14.14±0.53
		62.50	10.43±0.33	12.49±0.35	10.63±0.30
		31.25	—	10.81±0.27	9.83±0.12
	乙酸乙酯 ethyl acetate	500.00	17.62±0.22	25.21±2.09	12.82±0.30
		250.00	16.50±0.80	24.41±1.74	11.76±0.38
		125.00	10.52±1.50	21.53±1.11	9.59±0.29
		62.50	—	15.65±0.52	—
		31.25	—	11.28±0.75	—
DMSO			—	—	—

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Component and Antibacterial Activity of Different Polar Parts of Cinnamon and Homologous Root Bark

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