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Chemistry and Industry of Forest Products ›› 2019, Vol. 39 ›› Issue (4): 91-99.doi: 10.3969/j.issn.0253-2417.2019.04.013

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Purification of Bletilla striata Polyphenols by Macroporous Resin and Its Antioxidant Activities in Vitro

Yan PENG1(),Kehua TANG1,2,*(),Aiwen DONG1   

  1. 1. Key Laboratory of Human Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China
    2. Zhangjiajie Herbal Technology Co., Ltd., Zhangjiajie 427000, China
  • Received:2019-04-03 Online:2019-08-25 Published:2019-08-23
  • Contact: Kehua TANG E-mail:2418726447@qq.com;tkhthllh@126.com
  • Supported by:


The static adsorption and desorption effects of ten kinds of macroporous resins on B. striata polyphenols crude extract were compared, and the optimal process of purifying with LSA-21 resin was discussed. Furthermore, the antioxidant activities of the pre- and post-purification B. striata polyphenols were tested in vitro. The results showed that, at room temperature, the optimal process parameters for adsorption of crude extract with LSA-21 resin were as follows:the mass concentration of polyphenol crude extract 0.4-0.6 g/L, pH vlaue 4, and the loading velocity 2.0 mL/min. The optimal process parameters for desorption were as follows:desorption solution ethanol volume fraction 80%, pH vlaue 6, and elution flow rate 1.0 mL/min. The purity increased from 4.48% to 27.61% after purifying 100 mL of 0.47g/L crude extract under the optimal technological conditions, and the purification effect was good. The DPPH·scavenging activity of the purified B. striata polyphenols was obviously improved. The IC50 of DPPH·、OH· and O2-·free radicals were 7.72, 8.65 and 205.39 mg/L respectively.

Key words: Bletilla striata, polyphenols, macroporous resin, purification, antioxidation

CLC Number: