肥皂荚皂苷提取工艺优化及其表面张力研究

doi:10.3969/j.issn.0253-2417.2019.06.014

Abstract

Abstract:

The water extraction process of saponins from soap pods was optimized by single factor test and response surface method.The surface activity of saponins was analyzed and the structure of saponins was preliminarily characterized. The optimum extraction conditions were as follows:peel powder of soap pods 2.0 g, temperature 69℃, time 11 h 15 min, and the ratio of liquid to solid is 10:1 (mL:g). Under the above conditions, the yield of saponin is 26.49%. The purified saponins of soap pods were separated by 60% ethanol and 90% ethanol elution. The surface tensions were 47.43 and 35.83 m N/m, respectively. The critical micelle concentrations were 0.35 and 0.75 g/L, respectively. The total sugar content of the hydrolysis was 58.46% and 51.16%, respectively. The monosaccharides in the two kinds of saponins include glucose, xylose, rhamnose and arabinose, but the content of monosaccharide is slightly different. The maximum ultraviolet absorption wavelength of two kinds of saponins was about 470 nm. The infrared spectrum analysis shows that the two kinds of saponins both have the basic characteristic peaks of saponins.

Key words: Gleditsia saponin, water extraction, response surface methodology, surface tension, structural characterization

CLC Number:

TQ35

Zhexuan LÜ,Weiwei ZHANG,Dandan LIU,Liwei ZHU,Jianxin JIANG. Extraction Technology and Surface Tension Characterization of Gymnocladus chinensis Saponin[J]. Chemistry and Industry of Forest Products, 2019, 39(6): 102-108.

Figures/Tables 7

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Table 1

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References 24

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方差来源 sources of variance	平方和 sum of squares	自由度 df	均方 mean square	F值 F value	P值 P value	显著性¹⁾ significance
模型model	9.02	9	1.00	13.85	0.0023	**
X₁	0.070	1	0.070	0.97	0.3623
X₂	0.058	1	0.058	0.80	0.4058
X₃	3.613×10^-3	1	3.613×10^-3	0.050	0.8306
X₁X₂	1.38	1	1.38	19.08	0.0047	**
X₁X₃	8.100×10^-3	1	8.100×10^-3	0.11	0.7493
X₂X₃	0.17	1	0.17	2.38	0.1738
X₁²	1.64	1	1.64	22.74	0.0031	**
X₂²	2.71	1	2.71	37.52	0.0009	***
X₃²	2.97	1	2.97	41.01	0.0007	***
残差residual	0.43	6	0.072	3.45	0.1680
失拟项lack of fit	0.34	3	0.11
纯误差pure error	0.097	3	0.032
总和sum	9.45	15

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Extraction Technology and Surface Tension Characterization of Gymnocladus chinensis Saponin

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References 24

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序号 No.	X₁ 提取温度/℃ extraction temperature	X₂ 提取时间/h extraction time	X₃ 液料比值(mL:g) liquid-solid ratio	Y 得率/% yield
1	60	12	12	25.67
2	65	14	12	25.06
3	70	12	8	25.15
4	65	12	10	26.98
5	65	12	10	27.06
6	65	10	12	24.98
7	65	12	10	26.69
8	65	12	10	26.74
9	70	12	12	25.47
10	65	10	8	25.72
11	70	10	10	25.86
12	60	14	10	26.12
13	60	10	10	24.95
14	60	12	8	25.17
15	65	14	8	24.97
16	70	14	10	24.68