亚氯酸钠和氢氧化钠两步预处理杨木制备低聚木糖和单糖

doi:10.3969/j.issn.0253-2417.2021.05.004

Abstract

Abstract:

In this work, the effects of sodium chlorite(SC) and sodium hydroxide(SH) pretreatments on the chemical composition of poplar were investigated. The preparation of xylooligosaccharides(XOS) and monosaccharides by enzymatic hydrolysis was discussed. The results showed that poplar lignin could be selectively removed by SC pretreatment. After 6% SC pretreatment at 70℃ for 3 h, the removal of lignin reached 65.8%, whereas 90.7% glucan and 92.4% xylan were retained. The data of X-ray diffraction and X-ray photoelectron spectroscopy indicated that SC pretreatment removed the total lignin in poplar and the surface lignin on poplar, and increased the crystallinity and accessibility of the substrate. After the pretreatment with 6% SC at 70℃ and 1% SH at 50℃, the contents of glucan, xylan, and lignin in the pretreated poplar were 67.8%, 21.5%, and 2.9%, respectively. The highest XOS yield of 47.2% from the SC-SH-pretreated poplar by xylanase hydrolysis was obtained, and the XOS preparation was composed of 98% xylobiose. Compared with the poplar treated by 6% SC at 70℃ for 3 h, SH pretreatment increased the yield of XOS by 7.1 times. After the hydrolysis by xylanase, the glucose yield of two-step pretreated poplar residue was 90.2% with a cellulase lading of 30 mg/g dry matter. Finally, 52.5 g XOS, 317.6 g glucose and 49.9 g xylose were obtained from 1 kg raw poplar. The results of this work provided a theoretical guidance for the preparation of XOS and monosaccharides from poplar under mild conditions.

Key words: poplar, sodium chlorite, sodium hydroxide, xylooligosaccharides, enzymatic hydrolysis

CLC Number:

TQ35

Xin FANG, Peiyao WEN, Yong XU, Junhua ZHANG. Production of Xylooligosaccharides and Monosaccharides from Poplar by Two-step Sodium Chlorite and Sodium Hydroxide Pretreatment[J]. Chemistry and Industry of Forest Products, 2021, 41(5): 23-30.

Figures/Tables 8

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References 17

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样品 samples	预处理时间/h pretreatment time	葡聚糖/% glucan	木聚糖/% xylan	木质素/% lignin	乙酰基/% acetyl	固形物回收率/% solid recovery	移除率removal rate/%
样品 samples	预处理时间/h pretreatment time	葡聚糖/% glucan	木聚糖/% xylan	木质素/% lignin	乙酰基/% acetyl	固形物回收率/% solid recovery	葡聚糖 glucan	木聚糖 xylan	木质素 lignin
未处理untreated		40.0±0.8	17.8±0.6	28.0±0.6	4.0±1.0	100.0	0.0	0.0	0.0
SC_0.5	0.5	40.9±0.9	17.7±0.1	22.9±0.4	4.2±0.9	89.0±1.1	8.9	11.4	27.4
SC₁	1	42.5±0.5	18.9±0.4	20.5±0.4	4.2±1.7	87.0±0.8	7.6	7.7	36.3
SC₃	3	42.9±1.4	19.4±0.2	11.3±0.5	4.2±0.9	84.6±0.7	9.3	7.6	65.8

样品 samples	氧碳比 O/C	C/%			结晶度/% crystallinity(I_Cr)	最大吸附量/(mg·g^-1) maximum adsorption (Γ_{max, DR28})
样品 samples	氧碳比 O/C	C—C/C—H	C—O	C＝O/O—C—O	结晶度/% crystallinity(I_Cr)	最大吸附量/(mg·g^-1) maximum adsorption (Γ_{max, DR28})
未处理untreated	0.34±0.0	49.3±1.2	40.4±0.8	10.3±1.0	51.2±0.9	240.4
SC_0.5	0.53±0.0	20.2±0.9	47.2±1.1	32.6±0.7	55.0±1.6	259.3
SC₁	0.55±0.0	36.6±0.9	48.0±1.0	15.4±1.0	56.4±1.5	355.1
SC₃	0.45±0.0	67.2±1.3	22.5±1.9	10.3±0.8	56.5±0.6	566.5

样品 samples	葡聚糖 glucan	木聚糖 xylan	木质素 lignin	乙酰基 acetyl	固形物回收率 solid recovery	移除率removal
样品 samples	葡聚糖 glucan	木聚糖 xylan	木质素 lignin	乙酰基 acetyl	固形物回收率 solid recovery	葡聚糖 glucan	木聚糖 xylan	木质素 lignin
SC_0.5-SH	57.0±0.8	20.2±0.1	10.6±0.4	0	69.3±1.1	3.4	21.1	67.9
SC₁-SH	64.0±0.4	21.4±0.4	4.7±0.2	0	65.8±0.1	0.8	25.5	85.4
SC₃-SH	67.8±1.2	21.5±0.2	2.9±0.5	0	61.4±0.3	2.9	32.0	84.2

样品 samples	低聚木糖得率yield of xylooligosaccharides/%					总得率/% total yield	木糖与低聚木糖质量比值 mass ratio of xylose and XOS
样品 samples	木二糖 xylobiose	木三糖 xylotriose	木四糖 xylotetraose	木五糖 xylopentaose	木六糖 xylohexaose	总得率/% total yield	木糖与低聚木糖质量比值 mass ratio of xylose and XOS
SC_0.5	0.40±0.00	0.16±0.02	0.26±0.00	0.08±0.00	0.16±0.00	1.06±0.07	0.03
SC₁	0.42±0.01	0.33±0.00	0.62±0.01	0.20±0.01	0.03±0.00	1.60±0.09	0.42
SC₃	2.06±0.05	0.83±0.00	1.49±0.12	0.62±0.00	0.85±0.02	5.85±0.23	0.33
SC_0.5-SH	41.00±0.06	0.42±0.01	0.11±0.00	0.04±0.00	0.05±0.00	41.62±1.56	0.19
SC₁-SH	43.19±0.54	0.37±0.02	0.07±0.01	0.05±0.00	0.06±0.00	43.74±1.02	0.17
SC₃-SH	46.97±0.12	0.05±0.00	0.07±0.00	0.05±0.00	0.06±0.00	47.20±0.74	0.20

样品 samples	酶用量/(mg·g^-1) cellulase	葡萄糖得率/% glucose yield	木糖得率/% xylose yield
SC_0.5-SH	10	41.1±0.2	12.1±0.4
SC_0.5-SH	30	81.4±0.1	28.5±0.4
SC₁-SH	10	55.5±0.1	20.0±0.9
SC₁-SH	30	84.0±0.7	32.1±0.8
SC₃-SH	10	64.7±0.5	22.4±0.3
SC₃-SH	30	90.2±1.1	35.3±0.9

Production of Xylooligosaccharides and Monosaccharides from Poplar by Two-step Sodium Chlorite and Sodium Hydroxide Pretreatment

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