天然低共熔溶剂提取杜仲叶绿原酸及其抗氧化活性

doi:10.3969/j.issn.0253-2417.2022.01.014

Abstract

Abstract:

Chlorogenic acid was extracted from Eucommia ulmoides leaves by natural deep eutectic solvent (NADES) with ultrasonic assistance.Its antioxidant activity was investigated.Firstly, the better natural deep eutectic solvent system was screened, and then based on the single factor test, the extraction process was optimized through the response surface and the antioxidant activity of chlorogenic acid from E.ulmoides leaves was investigated.The results showed that the better solvent system was composed of betaine, L-lactic acid and water, and the ratio of each component was 1:1:4, and water consumption in eutectic solvent was 5 mol.The optimum conditions for E.ulmoides chlorogenic acid extract were liquid-to-material ratio of 30:1(mL: g), extraction temperature 40℃, extraction time 42 min, ultrasonic power 340 W.Under these conditions, the yield of chlorogenic acid was 31.46 mg/g, which increased by 18.23% and 14.82% respectively compared with the extraction solvent of water (26.61 mg/g) or 60% ethanol (27.40 mg/g).The antioxidant activity of chlorogenic acid extract from E.ulmoides leaves was studied in vitro.The results showed that the NADES extract had strong scavenging ability to DPPH·, ABTS·, and·OH, with IC₅₀ of 75.16, 19.98 and 314.20 mg/L, respectively.Its antioxidant capacity was stronger than those of water extract and alcohol extract, but slightly lower than that of Vc.

Key words: Eucommia ulmoides leaves, chlorogenic acid, natural deep eutectic solvents, response surface, antioxidant

CLC Number:

TQ35

Feng YU, Zhihong WANG, Guangyao ZHANG, Zhiyun DU, Mijun PENG. Extraction of Chlorogenic Acid From Eucommia ulmoides Leaves by Natural Deep Eutectic Solvent and Its Antioxidant Activity[J]. Chemistry and Industry of Forest Products, 2022, 42(1): 101-109.

Figures/Tables 6

Table 1

Fig.1

Table 2

Table 3

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References 15

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甜菜碱用量/mol betaine amount	L-乳酸用量/mol L-lactic amount	水用量/mol water amount	绿原酸得率/(mg·g^-1) chlorogenic acid yield
2	1	4	21.46
2	2	4	24.48
1	1	4	31.27
1	2	4	30.94
1	3	4	30.07

方差来源 source	平方和 SS	自由度 df	均方 MS	F值 F value	P值 P value	显著性 significance
模型model	27.47	9	3.05	35.42	< 0.0001	**
X₁	2.69	1	2.69	31.22	0.0008	**
X₂	0.987	1	0.987	11.45	0.0117	*
X₃	17.43	1	17.43	202.27	<0.0001	*
X₁X₂	0.731	1	0.731	8.48	0.0226	*
X₁X₃	0.087	1	0.087	1.01	0.3485
X₂X₃	0.81	1	0.81	9.4	0.0182	*
X₁²	0.4839	1	0.4839	5.61	0.0497	*
X₂²	0.629	1	0.629	7.3	0.0306	*
X₃²	3.23	1	3.23	37.53	0.0005	**
残差residual	0.6034	7	0.0862
失拟项lack of fit	0.2401	3	0.08	0.8811	0.5223
纯误差pure error	0.3633	4	0.0908
总和cor total	28.08	16

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Extraction of Chlorogenic Acid From Eucommia ulmoides Leaves by Natural Deep Eutectic Solvent and Its Antioxidant Activity

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序号 run	X₁ 提取时间/min extraction time	X₂ 超声波功率/W ultrasonic power	X₃ NADES中水用量/mol water amount in NADES	Y 绿原酸得率/(mg·g^-1) chlorogenic acid yield
1	10	300	4	28.23
2	30	500	2	28.26
3	30	500	6	30.51
4	30	400	4	30.78
5	10	500	4	30.07
6	10	400	2	27.10
7	50	300	4	30.20
8	30	300	6	30.40
9	30	400	4	30.58
10	30	400	4	30.10
11	10	400	6	30.15
12	50	400	6	31.05
13	30	400	4	30.14
14	30	300	2	26.94
15	30	400	4	30.59
16	50	400	2	28.59
17	50	500	4	30.34