Chemistry and Industry of Forest Products ›› 2022, Vol. 42 ›› Issue (5): 61-68.doi: 10.3969/j.issn.0253-2417.2022.05.009
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Xuhua HE1, Zhijiao SHI1, Xiaowei PENG1, Huan KAN1, Ping ZHAO2, Yun LIU1,*()
Received:
2021-07-30
Online:
2022-10-28
Published:
2022-11-02
Contact:
Yun LIU
E-mail:liuyun0402001@163.com
CLC Number:
Xuhua HE, Zhijiao SHI, Xiaowei PENG, Huan KAN, Ping ZHAO, Yun LIU. Antioxidant Activities Analysis of Different Polar Solvent Extracts from Camellia fascicularis H. T. Chang[J]. Chemistry and Industry of Forest Products, 2022, 42(5): 61-68.
Table 1
Contents of polyphenols and total flavonoids in different polar extracts from C. fascicularis"
样品 sample | 多酚/(mg·g-1) polyphenols | 总黄酮/(mg·g-1) total flavonoids |
石油醚相 petroleum ether phase | 90.181±3.384c | 50.500±2.508d |
氯仿相 chloroform phase | 84.414±2.298c | 75.148±3.792c |
乙酸乙酯相 ethyl acetate phase | 124.578±2.132b | 92.844±6.021b |
正丁醇相 n-butanol phase | 62.866±2.278d | 26.484±3.047e |
水相 water phase | 140.259±5.989a | 102.324±1.095a |
Table 2
The equation curve and IC50 value of the free radical scavenging rate and reducing power of different polar extracts from C. fascicularis"
样品 sample | 自由基 free radicals | 拟合方程 fitting equation | R2 | IC50/(mg·L-1) |
Vc | DPPH· | Y=-0.003 24X2+0.123 15X-0.060 47 | 0.964 | 5.287±0.309 |
·ABTS+ | Y=(-2.00×10-5)X2+0.009 20X-0.013 01 | 0.978 | 64.928±0.725 | |
·OH | Y=-0.082 57X2+0.457 73X-0.372 86 | 0.995 | 293.290±36.077 | |
总还原能力 reducing power | Y=(-2.26×10-6)X2+0.006 28X-0.174 21 | 0.986 | ||
水相 water phase | DPPH· | Y=(-6.08×10-5)X2+0.015 15X+0.005 54 | 0.989 | 38.634±2.556 |
·ABTS+ | Y=(-4.89×10-7)X2+0.001 31X+0.175 35 | 0.966 | 276.610±8.416 | |
·OH | Y=-0.006 17X2+0.114 40X+0.244 12 | 0.990 | 2 601.500±164.756 | |
总还原能力 reducing power | Y=(-3.20×10-7)X2+0.001 83X+0.033 13 | 0.992 | ||
乙酸乙酯相 ethyl acetate phase | DPPH· | Y=(-2.55×10-5)X2+0.009 40X+0.087 72 | 0.992 | 50.845±3.985 |
·ABTS+ | Y=(-4.15×10-7)X2+0.001 23X+0.108 67 | 0.998 | 362.754±22.555 | |
·OH | Y=-0.005 9X2+0.087 91X+0.318 74 | 0.954 | 2 472.160±826.007 | |
总还原能力 reducing power | Y=(-1.70×10-7)X2+0.001 15X+0.019 53 | 0.999 | ||
氯仿相 chloroform phase | DPPH· | Y=(-3.98×10-6)X2+0.004 77X+0.212 75 | 0.984 | 63.598±3.854 |
·ABTS+ | Y=(-1.45×10-7)X2+0.000 67X+0.131 69 | 0.998 | 643.692±29.651 | |
·OH | Y=-0.020 21X2+0.248 14X+0.245 28 | 0.959 | 1 130.630±157.932 | |
总还原能力 reducing power | Y=(-2.39×10-8)X2+(5.94×10-4)X+0.092 12 | 0.995 | ||
正丁醇相 n-butanol phase | DPPH· | Y=(-1.30×10-5)X2+0.006 00X-0.032 66 | 0.993 | 119.810±8.207 |
·ABTS+ | Y=(-1.22×10-7)X2+0.000 64X+0.038 72 | 0.996 | 865.188±35.706 | |
·OH | Y=-0.007 82X2+0.140 23X+0.069 13 | 0.999 | 3 936.230±621.700 | |
总还原能力 reducing power | Y=(6.92×10-10)X2+(4.18×10-4)X+0.097 8 | 0.998 | ||
石油醚相 petroleum ether phase | DPPH· | Y=(-1.04×10-5)X2+0.008 41X-0.011 07 | 0.998 | 66.197±5.950 |
·ABTS+ | Y=(-3.65×10-8)X2+0.000 40X+0.054 09 | 0.998 | 1 276.462±39.405 | |
·OH | Y=-0.076 52X2+0.442 93X+0.422 39 | 0.993 | 180.880±49.568 | |
总还原能力 reducing power | Y=(2.07×10-8)X2+(2.64×10-4)X+0.097 57 | 0.993 |
Table 3
Correlation analysis between the antioxidant capacities and contents of polyphenols and total flavonoids of different polar extracts from C. fascicularis"
项目 item | IC50(DPPH·) | IC50(·ABTS+) | IC50(·OH) | 总还原能力 total reducing power | 多酚 polyphenols | 总黄酮 total flavonoids |
IC50(DPPH·) | 1 | 0.779** | 0.224 | -0.786** | -0.889** | -0.914** |
IC50(·ABTS+) | 0.779** | 1 | -0.161 | -0.971** | -0.704** | -0.794** |
IC50(·OH) | 0.224 | -0.161 | 1 | 0.133 | -0.121 | -0.201 |
总还原能力 reducing power | -0.786** | -0.971** | 0.133 | 1 | 0.864** | 0.832** |
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