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Chemistry and Industry of Forest Products ›› 2022, Vol. 42 ›› Issue (5): 61-68.doi: 10.3969/j.issn.0253-2417.2022.05.009

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Antioxidant Activities Analysis of Different Polar Solvent Extracts from Camellia fascicularis H. T. Chang

Xuhua HE1, Zhijiao SHI1, Xiaowei PENG1, Huan KAN1, Ping ZHAO2, Yun LIU1,*()   

  1. 1. College of Life Sciences, Southwest Forestry University, Kunming 650224, China
    2. Key Laboratory of State Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, China
  • Received:2021-07-30 Online:2022-10-28 Published:2022-11-02
  • Contact: Yun LIU E-mail:liuyun0402001@163.com

Abstract:

In this study, polyphenols and total flavonoids were extracted from Camellia fascicularis H. T. Chang using different polar solvents such as water, petroleum ether, chloroform, ethyl acetate, and n-butanol. The radical scavenging rate on DPPH·, ·ABTS+, and ·OH and the reducing power were used as indicators to analyze the antioxidant capacity of different polar solvents extracts from C. fascicularis. The results showed that extraction abilities for polyphenols and total flavonoids from C. fascicularis by different polar extract solvents and the antioxidant capacities of different polar extracts were different. The order of extraction abilities for polyphenols and total flavonoids were as follows: water>ethyl acetate>petroleum ether>chloroform>n-butanol and water>ethyl acetate>chloroform>petroleum ether>n-butanol, respectively. C. fascicularis showed good antioxidant activities, and there were some differences in antioxidant capacities of different polar extracts. The comprehensive antioxidant capacities of water and ethyl acetate phases were higher than that of chloroform, n-butanol, and petroleum ether phases. The water phase extract exhibited the strongest scavenging capacities for DPPH· and ·ABTS+, and the IC50 values were (38.634±2.556) and (276.610±8.416)mg/L, respectively, followed by the ethyl acetate phase((50.845±3.985) and (362.754±22.555) mg/L). The petroleum ether phase showed the strongest scavenging capacities for ·OH with an IC50 value of (180.88±49.568) mg/L, followed by the chloroform phase((1 130.630±157.932)mg/L). The water phase demonstrated the strongest total reducing power with an absorbance value of 2.281±0.103 at the mass concentration of different polar extracts of 1 750 mg/L, followed by the ethyl acetate phase(1.504±0.021).

Key words: Camellia fascicularis H. T. Chang, different polar extracts, polyphenols, total flavonoids, antioxidant capacity

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