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Chemistry and Industry of Forest Products ›› 2023, Vol. 43 ›› Issue (1): 79-88.doi: 10.3969/j.issn.0253-2417.2023.01.010

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Extraction, Isolation and Structure Identification of Pigment Compounds from Ginkgo biloba Leaves

Hong SHEN1,2(), Changwei ZHANG1,3, Hongxia CHEN1, Ran TAO1,3, Jianxin JIANG2, Chengzhang WANG1,3,*()   

  1. 1. Institute of Chemical Industry of Forest Products, CAF; Key Lab. of Biomass Energy and Material, Jiangsu Province; Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grassland Administration; National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass; Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing 210042, China
    2. College of Materials Science and Technology, Beijing Forestry University, Beijing 100083, China
    3. Research Institute of Forestry New Technology, CAF, Beijing 100091, China
  • Received:2021-12-07 Online:2023-02-28 Published:2023-02-28
  • Contact: Chengzhang WANG E-mail:2978963446@qq.com;wangczlhs@sina.com


Ethanol extracts of three samples were prepared from Ginkgo biloba L. fresh leaves(sample 1), dry leaves(sample 2) and old leaves(sample 3). HPLC-DAD-MS method was used to analyze the pigment compounds in three G. biloba leaves samples. The separation, purification and structure identification of ginkgo leaf pigment compounds in sample 2 and sample 3 were conducted by liquid-liquid extraction, silica gel column chromatography, HPLC preparative chromatography, etc. From the three samples, 24 kinds of pigment compounds in G. biloba leaves were preliminarily speculated, including 17 kinds of chlorophyll compounds and 7 kinds of carotenoid compounds. Ten monomer pigment compounds in G. biloba leaves were isolated and purified from ethanol extract of sample 2 and sample 3. They were identified as β-carotene (A), pheophytin a (B), pheophytin b (C), lutein (D), phy-purpurin 18a (E), pyropheophytin a (F), 132-OH-pheophytin a (G), 132-OH-pheophytin a' (H), 132-OH-pheophytin b (I), and 132-OH-pheophytin b' (J), respectively, by mass spectrometry, ultraviolet spectroscopy, infrared spectroscopy and proton nuclear magnetic resonance spectroscopy combined with literature comparison. Among them, E, F, G, H, I and J were isolated from G. biloba leaves for the first time.

Key words: Ginkgo biloba leaves, pigments, extraction, separation, structure identification

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