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Chemistry and Industry of Forest Products ›› 2023, Vol. 43 ›› Issue (1): 97-103.doi: 10.3969/j.issn.0253-2417.2023.01.012

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Quantification and Antioxidant Activity of Flavonoids in Bamboo Leaves Extract of Bambusa

Rongmiao AN, Ting YUAN, Xuefeng GUO()   

  1. NFGA Key Laboratory for Bamboo & Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing 100102, China
  • Received:2021-11-22 Online:2023-02-28 Published:2023-02-28
  • Contact: Xuefeng GUO E-mail:gxf71622@icbr.ac.cn


To explore the development and utilization of flavonoids in the leaves of the main bamboo species of Bambusa, extracts of leaves from ten bamboo species were obtained by extraction, separation and purification, using bamboo leaves from Bambusa as the materials. A HPLC method was established for the determination of 5 flavonoids in extract of bamboo leaves, and the antioxidant activities of those extracts were evaluated by DPPH and ABTS assays. The HPLC method for the determination of 5 flavonoids isoorientin, orientin, vitexin, isovitexin and luteoside in extract of bamboo leaves was using YMC-Pack ODS-A column(250 mm×4.6 mm, 5 μm) with 0.5% acetic acid/water(A) and acetonitrile(B) as the mobile phases, elution with 14% B for 40 min, the injection volume 10 μL, the flow rate 1 mL/min, the column temperature 30 ℃ and the detection wavelength at 340 nm. Under these conditions, the five flavonoids were completely separated within 40 min, the degree of separation was greater than 1.2, and the concentration had a good linear correlation with the peak area(R2>0.999). The recovery rate was 94.36%-105.37%, and the relative standard deviation(RSD) was 0.98%-3.48%. These results indicated that the method was stable and reliable. The total contents of 5 flavonoids in extract of leaves from 10 bamboo species of Bambusa measured under these conditions were 62.30-223.24 mg/g, and the mass fraction of bamboo leaves extract from B. multiplex f. fernleaf was the highest(223.24 mg/g). The half inhibitory mass fraction(IC50) values of DPPH· and ·ABTS+ were 28.58-44.37 mg/L and 8.58-11.07 mg/L, respectively, slightly higher than those of Vc and BHT. The bamboo species with better DPPH· scavenging rate were B. eutuldoides (IC50=28.58 mg/L), B. textilis (IC50=28.68 mg/L) and B. multiplex f. fernleaf (IC50=31.65 mg/L). The bamboo species with better ABTS·+ scavenging rate were B. multiplex f. fernleaf(IC50=8.58 mg/L), B. multiplex f. silverstripe(IC50=9.11 mg/L) and B. textilis (IC50=9.27 mg/L). The IC50 values of B. multiplex f. fernleaf and B. textilis of two kinds of radicals were lower, which were the two bamboo species with the best removal effect and the most value of development and utilization.

Key words: HPLC, Bambusa, bamboo leaves extract, flavonoids, antioxidant activity

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