Welcome to Chemistry and Industry of Forest Products,

Chemistry and Industry of Forest Products ›› 2023, Vol. 43 ›› Issue (1): 133-139.doi: 10.3969/j.issn.0253-2417.2023.01.017

Previous Articles     Next Articles

Comparison on Enzymatic Activity, Polysaccharide Structure and Rheological Properties of Gleditsia sinensis Lam. Seeds During Germination

Yingying PEI1, Wei XU1, Xinfeiran PEI1, Jiufang DUAN1, Diansong CHEN2, Jianxin JIANG1,*()   

  1. 1. College of Materials Science and Technology, Beijing Forestry University; Engineering Research Center of Forestry Biomass Materials and Energy, Beijing 100083, China
    2. Guangzhou Degu Personal Care Products Co., Ltd., Guangzhou 510880, China
  • Received:2021-11-08 Online:2023-02-28 Published:2023-02-28
  • Contact: Jianxin JIANG E-mail:jiangjx@bjfu.edu.cn


The germination process of Gleditsia sinensis seeds was simulated at 25 and 35 ℃, respectively. The activity of α-D-galactosidase in cotyledon and endosperm, the molecular weight, monosaccharide content and rheological properties of G. sinensis galactomannan were studied at different germination times. The results showed that at 25 ℃, the activity of α-D-galactosidase in the endosperm of G. sinensis fluctuated from 3.45 to 5.78 U/g(2-8 days after germination), and then rapidly increased to 86.12 U/g(14 days after germination). At 35 ℃, the activity of α-D-galactosidase in the endosperm of G. sinensis fluctuated from 3.31 to 9.24 U/g. The α-D-galactosidase enzyme activity in cotyledon of G. sinensis was higher than that in endosperm. At the early germination stage, the higher germination temperature(35 ℃) was more conductive to the degradation of galactomannan. With the prolongation of germination time, the molecular mass and viscosity of G. sinensis galactomannan gradually decreased, and the solution changed from pseudoplastic fluid to Newtonian fluid on day 12 at 25 ℃. The contents of mannose and galactose in G. sinensis galactomannan decreased gradually with the extension of germination time, and the mass ratio of mannose to galactomannan(M/G value) increased gradually. The M/G reached the maximum value of 3.31 on the 14th day of germination at 35 ℃, which was expected to be a substitute product for expensive locust bean gum galactomannan.

Key words: Gleditsia sinensis galactomannan, seeds germination, α-D-galactosidase, molecular weight, rheological properties

CLC Number: