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Chemistry and Industry of Forest Products ›› 2023, Vol. 43 ›› Issue (2): 12-18.doi: 10.3969/j.issn.0253-2417.2023.02.002

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Optimization of Ultrasonic-assisted Extraction Technology of Saponins from Gleditsia sinensis Thorn by RSM and Its Hypoglycemic Activity

Yong LIU, Caihong ZHANG, Pujun XIE, Yejun DENG, Lixin HUANG()   

  1. Institute of Chemical Industry of Forest Products, CAF; Key Lab. of Biomass Energy and Material, Jiangsu Province; Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grassland Administration; National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing 210042, China
  • Received:2022-01-18 Online:2023-04-28 Published:2023-04-26
  • Contact: Lixin HUANG E-mail:l_x_huang@163.com

Abstract:

On the basis of single factor experiment, response surface methodology(RSM) was used to optimize the ultrasonic-assisted extraction optimum process of saponins from Gleditsia sinensis Thorn(GST), and the inhibitory activities of the extracts on α-glucosidase and α-amylase were investigated under the optimal extraction conditions. The results showed that the optimal ultrasonic-assisted extraction conditions were solid-liquid ratio of 16:1(mL: g), ethanol volume fraction of 60%, ultrasonic time of 80 min and extraction temperature of 50℃. Under these conditions, the yield of total saponins was 13.28%±0.25%, and the IC50 value of α-glucosidase was (0.146±0.019) g/L, which was stronger than that of positive control acarbose with the median inhibitory concentration(IC50) value of (0.48±0.18) g/L. When the mass concentration of the extract was 2 g/L, the inhibition rate of α-amylase could reach 35.13%±0.58%, indicating that GST had the potential development into a drug for type II diabetes.

Key words: saponins of Gleditsia sinensis Thorn, response surface methodology, α-glucosidase, α-amylase

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