Welcome to Chemistry and Industry of Forest Products,

›› 2002, Vol. 22 ›› Issue (2): 26-30.

• 研究报告 • Previous Articles     Next Articles

STUDIES ON XYLITOL FERMENTATION OF CORN COBS HYDROLYSATE BY CANDIDA SP.

SUN Kun-shan, XIA Li-ming   

  1. Biotechnology Institute, Zhejiang University, Hangzhou 310027, China
  • Received:2001-09-10 Revised:1900-01-01 Online:2002-06-30 Published:2002-06-30

Abstract: The adaptation of Candida sp.can effectively enhance its resistance to the inhibitors in the hydrolysate of corn cobs,and therefore improves the xylose utilization efficiency and xylitol yield.At 30℃,hydrolysate produced by dilute acid pretreatment can be directly fermented after simply treated by Ca(OH)2 using the adapted yeast strain.The optimum fermentation conditions were as follows:inoculum size 10%(volume ratio),inoculum age 17 h,nitrogen source 1.5 g/L Bacto-yeast extract and 2.5 g/L Bacto-tryptone,volume charge 120 mL in 250 mL flasks,intitial pH value 5.5.Xylose utilization efficiency over 79.8% and xylitol yield higher than 62.4% were obtained respectively.This process can effectively reduce the pretreatment costs,showing broad prospects of applications.

Key words: xylose, xylitol, corn cob, hydrolysate, fermentation

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