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Chemistry and Industry of Forest Products ›› 2016, Vol. 36 ›› Issue (2): 15-22.doi: 10.3969/j.issn.0253-2417.2016.02.003

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Binding Ability of N-(2-Methylene Pyridine) Dehydroabietylamine Schiff Base and Its Copper Complex with Bovine Serum Albumin

WANG Ping-ping1, HUANG Zhi-xiang1, GAO Wei-lin1, ZHANG Jie1, XU Wu-shuang1, ZHANG Yu1, LIU Qing-bo2, LONG Jian-ying2, FEI Bao-li1   

  1. 1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China;
    2. College of Science, Nanjing Forestry University, Nanjing 210037, China
  • Received:2015-05-06 Online:2016-04-25 Published:2016-04-26

Abstract: The binding abilities of anticancer compounds N-(2-methylene pyridine) dehydroabietylamine Schiff bases (L) and its copper complex[Cu(L)2Br] Br(1) with bovine serum albumin (BSA) under simulated physiological conditions were investigated by UV absorption spectroscopy, fluorescence spectroscopy and circular dichroism (CD). The results indicated that the fluorescence quenching of BSA was a static process by 1, and a dynamic quenching by L. Binding constants(Ka), binding sites (n) and corresponding thermodynamic parameters (ΔG<0,ΔH>0 and ΔS>0) at different temperatures were calculated, respectively. The main binding force between BSA and the compounds was proved to be hydrophobic interaction. The binding reactions were spontaneous processes controlled by enthalpy and free energy. And the analysis of synchronous fluorescence spectroscopy indicated that the binding of compounds changed BSA conformation, and circular dichroism confirmed that the binding interaction obviously modified the secondary structure of BSA from the typical α-helix structure into twisted chain.

Key words: dehydroabietylamine Schiff base complex, bovine serum albumin, fluorescence, UV-vis, circular dichroism

CLC Number: