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Chemistry and Industry of Forest Products ›› 2017, Vol. 37 ›› Issue (6): 65-72.doi: 10.3969/j.issn.0253-2417.2017.06.009

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Chemical Component and Antibacterial Activity of Essential Oil from Myanmar Pterocarpus macrocarpus

CHEN Hongxia1, KANG Xiutang2, YOU Longjie2, WANG Chengzhang1,3, YE Jianzhong1   

  1. 1. Institute of Chemical Industry of Forestry Products, CAF;National Engineering Lab. for Biomass Chemical Utilization;Key and Open Lab. of Forest Chemical Engineering, SFA;Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
    2. Fujian Quality Inspection Center of Incense Products, Quanzhou 362600, China;
    3. Research Institute of Forestry New Technology, CAF, Beijing 100091, China
  • Received:2017-04-11 Online:2017-12-25 Published:2018-01-05

Abstract: The essential oils of Myanmar Pterocarpus macrocarpus Kurz were obtained by using steam distillation(SD), solvent thermal extraction(STE) and subcritical fluid extraction(SFE), with the comparative analysis of the chemical composition of three methods. The results showed that SFE exhibited the highest essential oil yield of 1.95%, SD exhibited the lowest essential oil yield of 1.03%, the yield of STE was 1.74%. About 38 constituents of the essential oil obtained by SD were identified by GC-MS analysis with the main compounds of β-eudesmol(69.897%) and machilol(18.055%). About 48 constituents of the essential oil obtained by STE were identified with the main compounds of β-eudesmol(50.907%), homopterocarpin(13.322%), machilol(9.333%), 1-(1,1-dimethylethyl)-4-methoxy-benzene(2.481%) and espatulenol(2.285%). About 48 constituents of the essential oil obtained by SFE were identified with the main compounds of β-eudesmol(47.786%), homopterocarpin(13.516%), dimethyl 2,3-dicyano-2-butenedioate(5.429%), machilol(5.232%), 1-(1,1-dimethylethyl)-4-methoxy-benzene(2.699%) and espatulenol(2.636%). The antimicrobial activity of the essential oils obtained by threee extraction methods were tested by zone of inhibition test, MIC and MBC assays. The essential oil obtained by SD showed no antimicrobial activity against Klebsiella pneumoniae, Candida albicans and Bacillus subtilis. However, it showed higher antimicrobial activity with the MIC values against the Klebsiella pneumoniae, Candida albicans and Bacillus subtilis of 6.25, 25 and 6.25 g/L, respectively.

Key words: Pterocarpus macrocarpus Kurz, essential oil, steam distillation, subcritical fluid extraction, antibacterial activity

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