Chemistry and Industry of Forest Products ›› 2019, Vol. 39 ›› Issue (2): 122-128.doi: 10.3969/j.issn.0253-2417.2019.02.018
Xiyu QUAN,Pai PENG,Peiyao WEN,Jinjin WEI,Junhua ZHANG*()
Received:
2018-11-13
Online:
2019-04-25
Published:
2019-05-08
Contact:
Junhua ZHANG
E-mail:junhuazhang@nwafu.edu.cn
Supported by:
CLC Number:
Xiyu QUAN,Pai PENG,Peiyao WEN,Jinjin WEI,Junhua ZHANG. Extraction and Properties Analysis of Polysaccharides, Extractum and Gutta-percha from Eucommia ulmoides Oliver Leaves[J]. Chemistry and Industry of Forest Products, 2019, 39(2): 122-128.
Table 1
Chemical contents of E. ulmoides leaves"
级分 | 乙醇抽提物/% | 纤维素/% | 木聚糖/% | 酸不溶木质素+胶/% | 酸溶木质素/% | 蛋白质/% |
type | alcohol extract | cellulose | xylan | acid-insoluble lignin + gutta-percha | acid solublelignin | protein |
≤0.25 mm | 33.24±0.17 | 4.32±0.10 | 3.34±0.04 | 23.46±1.25 | 7.02±0.09 | 9.84±0.10 |
>0.25 mm | 33.48±0.41 | 5.91±0.19 | 3.63±0.19 | 22.15±0.82 | 5.76±0.36 | 10.65±0.06 |
Table 2
Effects of extraction conditions on yields of chemical constituents from leaves of E. ulmides"
温度/℃ | 料液比(g:mL) | 总黄酮/(mg·g-1) | 绿原酸/(mg·g-1) | 桃叶珊瑚苷/(mg·g-1) | 京尼平苷酸/(mg·g-1) |
temperature | solid-liquid ratio | total flavonoids | chlorogenic acid | aucubin | geniposidic acid |
1:6 | 19.36±1.27 | 2.32±0.36 | 1.30±0.24 | 0.58±0.08 | |
60 | 1:8 | 23.33±0.13 | 2.61±0.50 | 1.81±0.08 | 0.95±0.07 |
1:10 | 32.90±1.06 | 3.45±0.56 | 1.38±0.20 | 0.60±0.05 | |
1:6 | 29.81±0.26 | 3.43±0.04 | 1.65±0.00 | 0.72±0.09 | |
70 | 1:8 | 32.74±1.79 | 4.04±0.17 | 2.12±0.39 | 0.39±0.02 |
1:10 | 33.29±0.24 | 5.57±0.03 | 1.48±0.10 | 0.85±0.00 | |
1:6 | 32.79±1.00 | 3.82±0.22 | 1.36±0.30 | 0.46±0.07 | |
80 | 1:8 | 37.06±2.37 | 4.11±0.54 | 1.65±0.24 | 0.86±0.09 |
1:10 | 50.08±0.63 | 6.83±0.70 | 0.98±0.20 | 0.16±0.05 |
Table 3
Effects of enzyme kind on gutta-percha yield and enzymatic hydrolysis of residues from E.ulmoides leaves"
酶种类 | 葡萄糖/(g·L-1) | 木糖/(g·L-1) | 杜仲胶得率/% |
enzymes | glucose | xylose | gutta-percha yield |
CEL | 11.34±0.07 | 1.62±0.01 | 2.07±0.03 |
PEC | 1.64±0.01 | 1.51±0.10 | 1.30±0.13 |
AMS | 1.84±0.01 | 1.55±0.11 | 1.22±0.01 |
CEL+PEC | 9.97±0.12 | 1.65±0.11 | 2.00±0.11 |
CEL+AMS | 12.01±0.11 | 1.82±0.07 | 1.82±0.03 |
AMS+PEC | 1.72±0.02 | 1.54±0.16 | 1.30±0.07 |
CEL+PEC+AMS | 10.57±0.07 | 1.63±0.10 | 1.98±0.22 |
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