碱-羟甲基化预处理对甘蔗渣酶解和发酵效率的影响

doi:10.3969/j.issn.0253-2417.2020.04.012

Abstract

Abstract:

To investigate the effects of formaldehyde dosage on improving enzymatic hydrolysis efficiency and ethanol fermentation yield of sugarcane bagasse, the sequential NaOH and hydroxymethylation pretreatment (AHP) were used to treat sugarcane bagasse (SCB) with different proportion of formaldehyde by hydroxymethylation reaction. The results showed that when SCB was treated by AHP with substrate and formaldehyde ratio of 1:1.5 for 2 h, the delignification rate was 55.68% and the mass fraction of cellulose was 67.20%.Under the cellulase dosage of 20 FPIU/g(based on the mass of glucan), the 72 h glucose yield was 89.61%, which was 8.86 times higher than that of untreated SCB (9.09%). After 24 h fermentation, the ethanol concentration increased from 10.93 g/L of AP-SCB to 12.96 g/L of AHP-SCB-1.5.The structural features of the pretreated sugarcane bagasse were measured by FT-IR, and the hydrophobicity and cellulase accessibility were studied. The hydrophobicity (0.127 L/g) and enzymatic accessibility (505.73 mg/g) of AHP-SCB-1.5 were 64.64% and 1.11 times of AP-SCB, respectively. The results showed that alkali and hydroxymethylation had a synergistic effect on pretreatment process, which could improve the hydrophilicity and cellulase accessibility of materials.

Key words: hydroxymethylation, alkali pretreatment, sugarcane bagasse, enzymatic hydrolysis, fermentation

CLC Number:

TQ35

Yan JIN,Haiyan YANG,Zhengjun SHI,Dawei WANG,Gaofeng XU,Jing YANG. Effect of Alkali-hydroxymethylation Pretreatment on Sugarcane Bagasse Enzymatic Hydrolysis and Fermentation Efficiency[J]. Chemistry and Industry of Forest Products, 2020, 40(4): 86-92.

Figures/Tables 4

Table 1

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References 25

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样品¹⁾ sample	m(甘蔗渣):m(甲醛) m(SCB):m(HCHO)	葡聚糖/% glucan	木聚糖/% xylan	木质素/% lignin	木质素脱除率/% delignification
SCB		46.88±0.34	22.73±1.12	22.52±1.13
AP-SCB		66.83±1.34	23.31±1.17	11.52±0.57	48.85±2.34
AHP-SCB-1.5	1:1.5	67.20±1.36	22.67±1.13	9.98±0.50	55.68±0.98
AHP-SCB-3	1:3	68.10±1.41	23.05±1.15	9.12±0.46	59.50±0.67
AHP-SCB-5	1:5	69.50±1.48	23.31±1.17	8.76±0.44	61.10±2.16
HP-SCB-1.5	1:1.5	50.46±0.53	23.14±0.98	21.76±0.34	3.37±1.32
HP-SCB-3	1:3	53.36±0.45	22.86±1.19	20.68±0.59	8.17±1.54
HP-SCB-5	1:5	52.13±0.78	23.40±1.02	20.91±0.32	7.15±1.57

样品 sample	酶可及度/(mg·g^-1) enzyme accessibility	乙醇质量浓度/(g·L^-1) mass concentration of ethanol	乙醇得率/% ethanol yield
SCB	155.02±1.22
AP-SCB	455.61±1.13	10.93±0.54	74.71±1.13
AHP-SCB-1.5	505.73±1.04	12.96±0.35	82.27±1.05
AHP-SCB-3	502.26±2.47	11.44±0.57	80.39±2.47
AHP-SCB-5	467.51±1.58	9.28±0.46	64.76±1.58
HP-SCB-1.5	202.48±3.56	1.12±0.11	68.54±1.24
HP-SCB-3	202.46±2.47	1.14±0.21	65.13±0.76
HP-SCB-5	219.29±0.98	1.07±0.17	66.13±1.26

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Effect of Alkali-hydroxymethylation Pretreatment on Sugarcane Bagasse Enzymatic Hydrolysis and Fermentation Efficiency

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